Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry | |
| Jing, Nianhua1,3; Shi, Junyou2; Li, Guoliang4; Sun, Zhiwei4; You, Jinmao1,4 | |
| 2012-08-01 | |
| 发表期刊 | FOOD RESEARCH INTERNATIONAL
 |
| ISSN | 0963-9969 |
| 卷号 | 48期号:1页码:155-163 |
| 文章类型 | Article |
| 摘要 | Owing to the growing consumer interest in low-fat foods, it is necessary to provide our customers with the information about the fatty acid composition of mushrooms. In this study, a selective and sensitive method based on pre-column derivatization using 2-(12-oxobenzo[b]-acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) as the labeling reagent has been optimized by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS/MS). Fatty acids (FA) were derivatized by BAETS and separated on a reversed-phase Hypersil BDS C8 column with a gradient elution. Eighteen FA investigated were found to give excellent linear responses with correlation coefficients of >0.9996. Limits of detection and quantification (LOD and LOQ) were in the range of 0.46 to 1.02 ng mL(-1) and 1.43 to 3.48 ng mL(-1), respectively. This method was applied to the quantitative analysis of FA from a wild and four cultivated mushroom species. The wild mushroom named Armillaria luteo-virens contained higher unsaturated fatty acids (UFA) when compared to the cultivated species (Flammulina velutiper, Pleurotus eryngii, Copyinds comatus and Agrocybe aegerita). Ratio of UFA:SFA (SFA, saturated fatty acids) for A. luteo-virens was >5 whereas the values for the cultivated species were <4.09. Therefore, BAETS derivatization allowed the development of a highly sensitive and specific method for the determination of FA in mushroom samples. (C) 2012 Elsevier Ltd. All rights reserved.; Owing to the growing consumer interest in low-fat foods, it is necessary to provide our customers with the information about the fatty acid composition of mushrooms. In this study, a selective and sensitive method based on pre-column derivatization using 2-(12-oxobenzo[b]-acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) as the labeling reagent has been optimized by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS/MS). Fatty acids (FA) were derivatized by BAETS and separated on a reversed-phase Hypersil BDS C8 column with a gradient elution. Eighteen FA investigated were found to give excellent linear responses with correlation coefficients of >0.9996. Limits of detection and quantification (LOD and LOQ) were in the range of 0.46 to 1.02 ng mL(-1) and 1.43 to 3.48 ng mL(-1), respectively. This method was applied to the quantitative analysis of FA from a wild and four cultivated mushroom species. The wild mushroom named Armillaria luteo-virens contained higher unsaturated fatty acids (UFA) when compared to the cultivated species (Flammulina velutiper, Pleurotus eryngii, Copyinds comatus and Agrocybe aegerita). Ratio of UFA:SFA (SFA, saturated fatty acids) for A. luteo-virens was >5 whereas the values for the cultivated species were <4.09. Therefore, BAETS derivatization allowed the development of a highly sensitive and specific method for the determination of FA in mushroom samples. (C) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Fatty Acids Armillaria Luteo-virens Hplc-fld-ms/ms Pre-column Derivatization |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | WILD EDIBLE MUSHROOMS ; AMERICAN-HEART-ASSOCIATION ; ARMILLARIA-LUTEO-VIRENS ; CARDIOVASCULAR-DISEASE ; ELECTROSPRAY-IONIZATION ; GAS-CHROMATOGRAPHY ; NUTRITIONAL-VALUE ; AMINO-ACIDS ; IDENTIFICATION ; EXTRACTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Food Science & Technology |
| WOS类目 | Food Science & Technology |
| WOS记录号 | WOS:000307430100019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3632 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Qujing Normal Univ, Coll Chem Sci, Qujing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jing, Nianhua,Shi, Junyou,Li, Guoliang,et al. Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry[J]. FOOD RESEARCH INTERNATIONAL,2012,48(1):155-163. |
| APA | Jing, Nianhua,Shi, Junyou,Li, Guoliang,Sun, Zhiwei,&You, Jinmao.(2012).Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry.FOOD RESEARCH INTERNATIONAL,48(1),155-163. |
| MLA | Jing, Nianhua,et al."Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry".FOOD RESEARCH INTERNATIONAL 48.1(2012):155-163. |
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