Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Molecular identification of Echinococcus species from eastern and southern Qinghai, China, based on the mitochondrial cox1 gene | |
| Ma, Junying2; Wang, Hu2; Lin, Gonghua1; Craig, Philip S.3; Ito, Akira4; Cai, Zhenyuan1; Zhang, Tongzuo1; Han, Xiumin; Ma, Xiao2; Zhang, Jingxiao2; Liu, Yufang2; Zhao, Yanmei2; Wang, Yongshun2 | |
| 2012-07-01 | |
| 发表期刊 | PARASITOLOGY RESEARCH
 |
| ISSN | 0932-0113 |
| 卷号 | 111期号:1页码:179-184 |
| 文章类型 | Article |
| 摘要 | The Qinghai-Tibetan Plateau (QTP, in western China), which is the largest and highest plateau on Earth, is a highly epidemic region for Echinococcus spp. We collected 70 Echinococcus samples from humans, dogs, sheep, yaks, plateau pikas, and voles in eastern and southern Qinghai and genotyped them using the mitochondrial DNA marker cytochrome oxidase subunit I gene and maximum parsimony and Bayesian reconstruction methods. Based on the 792-bp sequence matrix, we recorded 124 variable sites, of which, 115 were parsimony-informative. Thirty-four haplotypes (H1-H34) were detected, of which H1-H15, H16-H17, and H18-H34 belonged to Echinococcus shiquicus, Echinococcus multilocularis, and Echinococcus granulosus, respectively. Within 26 human isolates, three were identified as E. multilocularis and 23 were E. granulosus. We also detected a dual infection case in a dog with E. multilocularis and E. granulosus. The intraspecific haplotype (Hd +/- SD) and nucleotide (Nd +/- SD) diversity of E. shiquicus (0.947 +/- 0.021; 0.00441 +/- 0.00062) was higher than that for E. granulosus (0.896 +/- 0.038; 0.00221 +/- 0.00031) and E. multilocularis (0.286 +/- 0.196; 0.00036 +/- 0.00025). Moreover, the haplotype network of E. shiquicus showed a radial feature rather than a divergent feature in a previous study, indicating this species in the QTP has also evolved with bottleneck effects.; The Qinghai-Tibetan Plateau (QTP, in western China), which is the largest and highest plateau on Earth, is a highly epidemic region for Echinococcus spp. We collected 70 Echinococcus samples from humans, dogs, sheep, yaks, plateau pikas, and voles in eastern and southern Qinghai and genotyped them using the mitochondrial DNA marker cytochrome oxidase subunit I gene and maximum parsimony and Bayesian reconstruction methods. Based on the 792-bp sequence matrix, we recorded 124 variable sites, of which, 115 were parsimony-informative. Thirty-four haplotypes (H1-H34) were detected, of which H1-H15, H16-H17, and H18-H34 belonged to Echinococcus shiquicus, Echinococcus multilocularis, and Echinococcus granulosus, respectively. Within 26 human isolates, three were identified as E. multilocularis and 23 were E. granulosus. We also detected a dual infection case in a dog with E. multilocularis and E. granulosus. The intraspecific haplotype (Hd +/- SD) and nucleotide (Nd +/- SD) diversity of E. shiquicus (0.947 +/- 0.021; 0.00441 +/- 0.00062) was higher than that for E. granulosus (0.896 +/- 0.038; 0.00221 +/- 0.00031) and E. multilocularis (0.286 +/- 0.196; 0.00036 +/- 0.00025). Moreover, the haplotype network of E. shiquicus showed a radial feature rather than a divergent feature in a previous study, indicating this species in the QTP has also evolved with bottleneck effects. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | TIBET PLATEAU REGION ; POLYMORPHISM ; SHIQUICUS ; PIKA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Parasitology |
| WOS类目 | Parasitology |
| WOS记录号 | WOS:000305532700019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3650 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Qinghai Inst Endem Dis Prevent & Control, Xining 811602, Peoples R China 3.Univ Salford, Sch Environm & Life Sci, Cestode Zoonoses Res Grp, Manchester M5 4WT, Lancs, England 4.Asahikawa Med Univ, Dept Parasitol, Asahikawa, Hokkaido 0788510, Japan |
| 推荐引用方式 GB/T 7714 | Ma, Junying,Wang, Hu,Lin, Gonghua,et al. Molecular identification of Echinococcus species from eastern and southern Qinghai, China, based on the mitochondrial cox1 gene[J]. PARASITOLOGY RESEARCH,2012,111(1):179-184. |
| APA | Ma, Junying.,Wang, Hu.,Lin, Gonghua.,Craig, Philip S..,Ito, Akira.,...&Wang, Yongshun.(2012).Molecular identification of Echinococcus species from eastern and southern Qinghai, China, based on the mitochondrial cox1 gene.PARASITOLOGY RESEARCH,111(1),179-184. |
| MLA | Ma, Junying,et al."Molecular identification of Echinococcus species from eastern and southern Qinghai, China, based on the mitochondrial cox1 gene".PARASITOLOGY RESEARCH 111.1(2012):179-184. |
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