Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling

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	Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling
	Song, Cuihua 1; Zhang, Caiqing 2; Li, Guoliang 1,3; Zhang, Xiaolong 4,5; Chen, Guang 3,4; You, Jinmao 1,3
	2012-03-01
发表期刊	INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
ISSN	0963-7486
卷号	63 期号:2 页码:170-177
文章类型	Article
摘要	Amino acids (AA) are important chemical constituents of tea leaves remarkably influencing the quality of tea. In this study, free AA and total AA in Apocynum venetum L. (Luobuma tea) were estimated by HPLC equipped with fluorescent detector using 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEEC) as a fluorescent labelling reagent. Different parameters for derivatization and separation were optimized. AA were rapidly derivatized within 3 min at room temperature with DBCEEC. In conjunction with a gradient elution, a baseline resolution of 20 analytes was achieved on a reversed-phase Hypersil BDS C18 column. LC separation for the derivatized AA showed good reproducibility. Twenty AA were detected and showed significant linear responses with correlation coefficients (>0.9992). This developed method offered the low detection limit of 2.88-23.4 fmol.; Amino acids (AA) are important chemical constituents of tea leaves remarkably influencing the quality of tea. In this study, free AA and total AA in Apocynum venetum L. (Luobuma tea) were estimated by HPLC equipped with fluorescent detector using 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEEC) as a fluorescent labelling reagent. Different parameters for derivatization and separation were optimized. AA were rapidly derivatized within 3 min at room temperature with DBCEEC. In conjunction with a gradient elution, a baseline resolution of 20 analytes was achieved on a reversed-phase Hypersil BDS C18 column. LC separation for the derivatized AA showed good reproducibility. Twenty AA were detected and showed significant linear responses with correlation coefficients (>0.9992). This developed method offered the low detection limit of 2.88-23.4 fmol.
关键词	Amino Acids Analysis Hplc-fld Analysis Pre-column Derivatization Apocynum Venetum L.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	LIQUID-CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; DERIVATIZATION ; MICROCHIPS
收录类别	SCI
语种	英语
WOS研究方向	Food Science & Technology ; Nutrition & Dietetics
WOS类目	Food Science & Technology ; Nutrition & Dietetics
WOS记录号	WOS:000299745600006
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3718
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 2.Shandong Prov Qianfoshan Hosp, Jinan 250021, Peoples R China 3.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 4.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Song, Cuihua,Zhang, Caiqing,Li, Guoliang,et al. Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling[J]. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION,2012,63(2):170-177.
APA	Song, Cuihua,Zhang, Caiqing,Li, Guoliang,Zhang, Xiaolong,Chen, Guang,&You, Jinmao.(2012).Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling.INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION,63(2),170-177.
MLA	Song, Cuihua,et al."Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling".INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION 63.2(2012):170-177.