Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing

NWIPB OpenIR

	Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing
	Zhao, J. ; Zhao, K. ; Peng, Z.
	2014-10-01
发表期刊	JOURNAL OF APPLIED ICHTHYOLOGY ; Zhao, J; Zhao, K; Peng, Z.Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing,JOURNAL OF APPLIED ICHTHYOLOGY,2014,30(5):1056
摘要	Triplophysa rosa is an endangered cave-dwelling and endemic fish species found only in Chongqing, southwestern China. The genetic data available for this species is very limited. Polymorphic microsatellites were identified in the genome of T. rosa using 454 sequencing. Of the 145 loci screened, 106 were amplified successfully and 11 showed polymorphic patterns. The number of alleles ranged from 2 to 6, and the observed and expected heterozygosity varied from 0.061 to 0.543 (mean = 0.349) and from 0.248 to 0.789 (mean = 0.551), respectively. The polymorphic information content (PIC) ranged from 0.215 to 0.744 (mean = 0.486), indicating moderate levels of polymorphism. In addition, cross-species amplification was tested for the 106 loci in Triplophysa moquensis, which showed a high level of transferability (76.4%), implying that the microsatellite markers developed here could be used effectively for other closely related species.; Triplophysa rosa is an endangered cave-dwelling and endemic fish species found only in Chongqing, southwestern China. The genetic data available for this species is very limited. Polymorphic microsatellites were identified in the genome of T. rosa using 454 sequencing. Of the 145 loci screened, 106 were amplified successfully and 11 showed polymorphic patterns. The number of alleles ranged from 2 to 6, and the observed and expected heterozygosity varied from 0.061 to 0.543 (mean = 0.349) and from 0.248 to 0.789 (mean = 0.551), respectively. The polymorphic information content (PIC) ranged from 0.215 to 0.744 (mean = 0.486), indicating moderate levels of polymorphism. In addition, cross-species amplification was tested for the 106 loci in Triplophysa moquensis, which showed a high level of transferability (76.4%), implying that the microsatellite markers developed here could be used effectively for other closely related species.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/37337
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, J.,Zhao, K.,Peng, Z.. Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing[J]. JOURNAL OF APPLIED ICHTHYOLOGY, Zhao, J; Zhao, K; Peng, Z.Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing,JOURNAL OF APPLIED ICHTHYOLOGY,2014,30(5):1056,2014.
APA	Zhao, J.,Zhao, K.,&Peng, Z..(2014).Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing.JOURNAL OF APPLIED ICHTHYOLOGY.
MLA	Zhao, J.,et al."Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing".JOURNAL OF APPLIED ICHTHYOLOGY (2014).