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PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC
Chen, Tao1,2; Wang, Ping1,2; Du, Yuzhi1; Shen, Yuhu1; Li, Yulin1
2012
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
卷号35期号:18页码:2524-2532
文章类型Article
摘要In order to isolate large amounts of pure lutonarin and saponarin, a high performance method for isolation and purification of these two flavonoids from barley (Hordeum vulgare) seedlings was successfully established by utilizing high-speed counter-current chromatography (HSCCC). Separation was performed with a two-phase solvent system: ethyl acetate/n-butanol/water (3:2:5, v/v/v). A total of 24 mg lutonarin and 14 mg saponarin was obtained in one-step separation from 100 mg sample. High performance liquid chromatography (HPLC) analysis showed that the purity of each of the two compounds was over 98%. Their chemical structures were confirmed by UV, H-1 NMR, and C-13 NMR data.; In order to isolate large amounts of pure lutonarin and saponarin, a high performance method for isolation and purification of these two flavonoids from barley (Hordeum vulgare) seedlings was successfully established by utilizing high-speed counter-current chromatography (HSCCC). Separation was performed with a two-phase solvent system: ethyl acetate/n-butanol/water (3:2:5, v/v/v). A total of 24 mg lutonarin and 14 mg saponarin was obtained in one-step separation from 100 mg sample. High performance liquid chromatography (HPLC) analysis showed that the purity of each of the two compounds was over 98%. Their chemical structures were confirmed by UV, H-1 NMR, and C-13 NMR data.
关键词Barley Seedlings Hordeum Vulgare Hplc Hsccc Lutonarin Saponarin
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]SPEED COUNTERCURRENT CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; HORDEUM-VULGARE ; LEAVES ; FLAVONOIDS ; GLYCOSIDES
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000310738000002
引用统计
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/3736
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Qinghai Prov, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
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GB/T 7714
Chen, Tao,Wang, Ping,Du, Yuzhi,et al. PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(18):2524-2532.
APA Chen, Tao,Wang, Ping,Du, Yuzhi,Shen, Yuhu,&Li, Yulin.(2012).PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,35(18),2524-2532.
MLA Chen, Tao,et al."PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 35.18(2012):2524-2532.
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