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| DETERMINATION AND IDENTIFICATION OF FATTY ACIDS IN MICROULA SIKKIMENSIS SEED OIL USING 1,2-BENZOCARBAZOLE-9-ETHYL-P-TOLUENESULFONATE AS A NOVEL LABELING REAGENT BY HPLC WITH FLUORESCENCE DETECTION AND APCI-MS | |
| Song, Cuihua1; Zhang, Caiqing2; Sun, Zhiwei1; You, Jinmao1,3; Suo, Yourui3 | |
| 2012 | |
| 发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| ISSN | 1082-6076 |
| 卷号 | 35期号:14页码:2066-2080 |
| 文章类型 | Article |
| 摘要 | Using 1,2-benzocarbazole-9-ethyl-p-toluenesulfonate (BCETS) as the labeling reagent, a sensitive method for the determination of fatty acids by high performance liquid chromatography (HPLC) with fluorescence detection has been developed. The BCETS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 30 min in N,N-dimethylformamide solvent. All the tested fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C-8 column by HPLC in conjunction with gradient elution. The identification was carried out by post-column atmospheric pressure chemical ionization mass spectrometry (APCI/MS) in positive ion detection mode. The fluorescence excitation and emission wavelengths of the derivatives were at lambda(ex) 279 nm and lambda(em) 380 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are more than 0.9994. Detection limits, at a signal-to-noise ratio of 3:1, were 0.21-0.99 mu g/L for the labeled fatty acids. In this study, fatty acids from the extracted Microula sikkimensis Hemsl (M. sikkimensis H.) seed were sensitively determined. It was found that the seed oil mainly contained Oleic acid, Linoleic acid, alpha-Linolenic acid, Hexadecanoic acid, Stearidonic acid, gamma-Linolenic acid, and Eicosenoic acid. M. sikkimensis H. seed oil was rich in unsaturated fatty acids and may be used as high-quality edible oil for its health benefits.; Using 1,2-benzocarbazole-9-ethyl-p-toluenesulfonate (BCETS) as the labeling reagent, a sensitive method for the determination of fatty acids by high performance liquid chromatography (HPLC) with fluorescence detection has been developed. The BCETS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 30 min in N,N-dimethylformamide solvent. All the tested fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C-8 column by HPLC in conjunction with gradient elution. The identification was carried out by post-column atmospheric pressure chemical ionization mass spectrometry (APCI/MS) in positive ion detection mode. The fluorescence excitation and emission wavelengths of the derivatives were at lambda(ex) 279 nm and lambda(em) 380 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are more than 0.9994. Detection limits, at a signal-to-noise ratio of 3:1, were 0.21-0.99 mu g/L for the labeled fatty acids. In this study, fatty acids from the extracted Microula sikkimensis Hemsl (M. sikkimensis H.) seed were sensitively determined. It was found that the seed oil mainly contained Oleic acid, Linoleic acid, alpha-Linolenic acid, Hexadecanoic acid, Stearidonic acid, gamma-Linolenic acid, and Eicosenoic acid. M. sikkimensis H. seed oil was rich in unsaturated fatty acids and may be used as high-quality edible oil for its health benefits. |
| 关键词 | Derivatization Fatty Acids Fluorescence Detection High Performance Liquid Chromatography Mass Spectrometry Microula Sikkimensis Hemsl Seed Oil |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; CARBOXYLIC-ACIDS ; TRIFLUOROMETHANESULFONATE ; BRYOPHYTE ; POLLEN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000308024300011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3744 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal Shandong Pr, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 2.Shandong Prov Qianfoshan Hosp, Jinan, Peoples R China 3.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Cuihua,Zhang, Caiqing,Sun, Zhiwei,et al. DETERMINATION AND IDENTIFICATION OF FATTY ACIDS IN MICROULA SIKKIMENSIS SEED OIL USING 1,2-BENZOCARBAZOLE-9-ETHYL-P-TOLUENESULFONATE AS A NOVEL LABELING REAGENT BY HPLC WITH FLUORESCENCE DETECTION AND APCI-MS[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(14):2066-2080. |
| APA | Song, Cuihua,Zhang, Caiqing,Sun, Zhiwei,You, Jinmao,&Suo, Yourui.(2012).DETERMINATION AND IDENTIFICATION OF FATTY ACIDS IN MICROULA SIKKIMENSIS SEED OIL USING 1,2-BENZOCARBAZOLE-9-ETHYL-P-TOLUENESULFONATE AS A NOVEL LABELING REAGENT BY HPLC WITH FLUORESCENCE DETECTION AND APCI-MS.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,35(14),2066-2080. |
| MLA | Song, Cuihua,et al."DETERMINATION AND IDENTIFICATION OF FATTY ACIDS IN MICROULA SIKKIMENSIS SEED OIL USING 1,2-BENZOCARBAZOLE-9-ETHYL-P-TOLUENESULFONATE AS A NOVEL LABELING REAGENT BY HPLC WITH FLUORESCENCE DETECTION AND APCI-MS".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 35.14(2012):2066-2080. |
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