Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY | |
| Jing, Nianhua1,3; Li, Guoliang1,3; Sun, Zhiwei1,3; You, Jinmao1,2 | |
| 2012 | |
| 发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| ISSN | 1082-6076 |
| 卷号 | 35期号:13页码:1882-1894 |
| 文章类型 | Article |
| 摘要 | Fatty acids (FA) are the basic and indispensable components in medicinal herbs. In the present work, a selective and sensitive method based on pre-column derivatization for the qualitative and quantitative analysis of FA from three snow lotus species (S. involucrata, S. laniceps, and S. medusa) was developed, and a comparative study based on the FA contents was carried out. FA were determined by HPLC with fluorescence detection, using 2-(12-oxobenzo[b]acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) as the labeling reagent and were identified with post-column APCI-MS. FA could be easily and quickly tagged by BAETS at 95 degrees C in the presence of K2CO3 catalyst in N, N'-dimethylformamide (DMF). All validation procedures including linearity, limits of detection and quantification, precision, accuracy, ruggedness, and robustness were performed, and the results showed that the novel method exhibited excellent reproducibility and applicability. This proposed method was successfully applied to the analysis of total fatty acids (FA) and free fatty acids (FA) from snow lotus species. The content of FA in S. laniceps was significantly lower in comparison to the other two species that had similar FA content. Furthermore, this developed method exhibited a powerful potential for accurate detection of FA from other medicinal herbs or food stuff.; Fatty acids (FA) are the basic and indispensable components in medicinal herbs. In the present work, a selective and sensitive method based on pre-column derivatization for the qualitative and quantitative analysis of FA from three snow lotus species (S. involucrata, S. laniceps, and S. medusa) was developed, and a comparative study based on the FA contents was carried out. FA were determined by HPLC with fluorescence detection, using 2-(12-oxobenzo[b]acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) as the labeling reagent and were identified with post-column APCI-MS. FA could be easily and quickly tagged by BAETS at 95 degrees C in the presence of K2CO3 catalyst in N, N'-dimethylformamide (DMF). All validation procedures including linearity, limits of detection and quantification, precision, accuracy, ruggedness, and robustness were performed, and the results showed that the novel method exhibited excellent reproducibility and applicability. This proposed method was successfully applied to the analysis of total fatty acids (FA) and free fatty acids (FA) from snow lotus species. The content of FA in S. laniceps was significantly lower in comparison to the other two species that had similar FA content. Furthermore, this developed method exhibited a powerful potential for accurate detection of FA from other medicinal herbs or food stuff. |
| 关键词 | a Comparative Study Baets Fatty Acid Analysis Hplc-apci-ms Pre-column Derivatization Saussurea Species |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; PHYLOGENETIC ANALYSES ; IDENTIFICATION ; ASTERACEAE ; BRYOPHYTE ; CARDUEAE ; MEDUSA ; LIPIDS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000307648600009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3746 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adapt & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jing, Nianhua,Li, Guoliang,Sun, Zhiwei,et al. QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(13):1882-1894. |
| APA | Jing, Nianhua,Li, Guoliang,Sun, Zhiwei,&You, Jinmao.(2012).QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,35(13),1882-1894. |
| MLA | Jing, Nianhua,et al."QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 35.13(2012):1882-1894. |
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