Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography | |
| Yue, Hui-Lan1; Zhao, Xiao-Hui1,2; Mei, Li-Juan1; Shao, Yun1; Zhao, XH (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China. | |
| 2013-09-01 | |
| 发表期刊 | JOURNAL OF SEPARATION SCIENCE
 |
| ISSN | 1615-9306 |
| 卷号 | 36期号:18页码:3123-3129 |
| 文章类型 | Article |
| 摘要 | Five phenylethanoid glycosides (PhGs), forsythoside B, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside B, were isolated and purified from Lamiophlomis rotata (Benth.) Kudo by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation. In the present study, the two-phase solvent system composed of ethyl acetate/n-butanol/water (13:3:10, v/v/v) was used for HSCCC separation. A total of 27 mg of forsythoside B, 41 mg of verbascoside, 29 mg of alyssonoside, 23 mg of isoverbascoside, and 13 mg of leucosceptoside B with purities of 97.7, 99.2, 99.5, 99.3, and 97.3%, respectively, were obtained in a one-step separation within 4 h from 150 mg of crude extract. The recoveries of the five PhGs after MR-HSCCC separation were 74.5, 76.5, 72.5, 76.4, and 77.0%, respectively. The chemical structures of all five compounds were identified by 1H and 13C NMR spectroscopy.; Five phenylethanoid glycosides (PhGs), forsythoside B, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside B, were isolated and purified from Lamiophlomis rotata (Benth.) Kudo by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation. In the present study, the two-phase solvent system composed of ethyl acetate/n-butanol/water (13:3:10, v/v/v) was used for HSCCC separation. A total of 27 mg of forsythoside B, 41 mg of verbascoside, 29 mg of alyssonoside, 23 mg of isoverbascoside, and 13 mg of leucosceptoside B with purities of 97.7, 99.2, 99.5, 99.3, and 97.3%, respectively, were obtained in a one-step separation within 4 h from 150 mg of crude extract. The recoveries of the five PhGs after MR-HSCCC separation were 74.5, 76.5, 72.5, 76.4, and 77.0%, respectively. The chemical structures of all five compounds were identified by 1H and 13C NMR spectroscopy. |
| 关键词 | Hsccc Lamiophlomis Rotata (Benth.) Kudo Macroporous Resins Phenylpropanoid Glycosides Traditional Chinese Medicines |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1002/jssc.201300268 |
| 关键词[WOS] | SCROPHULARIA NINGPOENSIS HEMSLEY ; PHENYLETHANOID GLYCOSIDES ; PREPARATIVE ISOLATION ; BALLOTA-NIGRA ; IDENTIFICATION ; VERBASCOSIDE ; ROTH ; ROOT |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Natural Science Foundation of China(21201196) ; West Light Foundation of the Chinese Academy of Sciences(Y329241211) |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000324379400020 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3927 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Zhao, XH (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Hui-Lan,Zhao, Xiao-Hui,Mei, Li-Juan,et al. Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(18):3123-3129. |
| APA | Yue, Hui-Lan,Zhao, Xiao-Hui,Mei, Li-Juan,Shao, Yun,&Zhao, XH .(2013).Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,36(18),3123-3129. |
| MLA | Yue, Hui-Lan,et al."Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 36.18(2013):3123-3129. |
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