Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| NOVEL REAGENT FOR THE SENSITIVE DETERMINATION OF FREE FATTY ACIDS BY HPLC WITH FLUORESCENCE DETECTION AND IDENTIFICATION WITH MASS SPECTROMETRY AND APPLICATION TO SEVERAL MEDICINAL HERBS | |
| Chen, Guang1,2,3,4; Wang, Chengyan3; You, Jinmao1,3,4; Song, Cuihua3,4; Sun, Zhiwei3,4; Li, Guoliang2,3; Kang, Li2,3; You, JM (reprint author), Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China. | |
| 2013-05-01 | |
| 发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| ISSN | 1082-6076 |
| 卷号 | 36期号:15页码:2107-2124 |
| 文章类型 | Article |
| 摘要 | A new fluorescence reagent 2-(2-(4-(dimethylamino)-phenyl)-1H-phenanthro-[9,10-d]- imidazol-1-yl)-ethyl-4-methylbenzenesulfonate (PPMB) has been designed for fatty acids labeling. Fifteen fatty acids containing six saturated and nine unsaturated fatty acids were used to evaluate the analytical potential of this reagent. The derivatization reaction of PPMB with fatty acids was carried out in the presence of K2CO3 catalyst at 95 degrees C for 40min in N, N-dimethylformamide solvent. The separation of fatty acids was achieved on a reversed-phase Hypersil BDS C-8 column (4.6x200mm, 5 mu m) with a good baseline resolution. The fluorescence excitation and emission wavelengths were set at (ex) 260nm and (em) 430nm, respectively. The identification was carried out by the online APCI-MS in positive-ion detection mode. Results showed the fatty acid derivatives not only enabled sensitive fluorescence detection but also had high MS ionizability. Excellent linear responses were observed using fluorescent detection with coefficients of >0.9993. Detection limits, at a signal-to-noise ratio of 3:1, were in the range of 29.88-69.37 fmol. Fatty acid extracts from Lycium chinese mill, Long pepper, and Evodiae fructus were successfully analyzed with satisfactory results.; A new fluorescence reagent 2-(2-(4-(dimethylamino)-phenyl)-1H-phenanthro-[9,10-d]- imidazol-1-yl)-ethyl-4-methylbenzenesulfonate (PPMB) has been designed for fatty acids labeling. Fifteen fatty acids containing six saturated and nine unsaturated fatty acids were used to evaluate the analytical potential of this reagent. The derivatization reaction of PPMB with fatty acids was carried out in the presence of K2CO3 catalyst at 95 degrees C for 40min in N, N-dimethylformamide solvent. The separation of fatty acids was achieved on a reversed-phase Hypersil BDS C-8 column (4.6x200mm, 5 mu m) with a good baseline resolution. The fluorescence excitation and emission wavelengths were set at (ex) 260nm and (em) 430nm, respectively. The identification was carried out by the online APCI-MS in positive-ion detection mode. Results showed the fatty acid derivatives not only enabled sensitive fluorescence detection but also had high MS ionizability. Excellent linear responses were observed using fluorescent detection with coefficients of >0.9993. Detection limits, at a signal-to-noise ratio of 3:1, were in the range of 29.88-69.37 fmol. Fatty acid extracts from Lycium chinese mill, Long pepper, and Evodiae fructus were successfully analyzed with satisfactory results. |
| 关键词 | Derivatization Evodiae Fructus Fatty Acids Hplc Long Pepper Lycium Chinese Mill |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; CARBOXYLIC-ACIDS ; DERIVATIZATION REAGENT ; TAGGING REAGENTS ; LABELING REAGENT ; EVODIAE FRUCTUS ; DERIVATIVES ; FRUITS ; 4-BROMOMETHYL-7-ACETOXYCOUMARIN ; SEPARATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000320019900005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3944 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | You, JM (reprint author), Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China 4.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Guang,Wang, Chengyan,You, Jinmao,et al. NOVEL REAGENT FOR THE SENSITIVE DETERMINATION OF FREE FATTY ACIDS BY HPLC WITH FLUORESCENCE DETECTION AND IDENTIFICATION WITH MASS SPECTROMETRY AND APPLICATION TO SEVERAL MEDICINAL HERBS[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2013,36(15):2107-2124. |
| APA | Chen, Guang.,Wang, Chengyan.,You, Jinmao.,Song, Cuihua.,Sun, Zhiwei.,...&You, JM .(2013).NOVEL REAGENT FOR THE SENSITIVE DETERMINATION OF FREE FATTY ACIDS BY HPLC WITH FLUORESCENCE DETECTION AND IDENTIFICATION WITH MASS SPECTROMETRY AND APPLICATION TO SEVERAL MEDICINAL HERBS.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,36(15),2107-2124. |
| MLA | Chen, Guang,et al."NOVEL REAGENT FOR THE SENSITIVE DETERMINATION OF FREE FATTY ACIDS BY HPLC WITH FLUORESCENCE DETECTION AND IDENTIFICATION WITH MASS SPECTROMETRY AND APPLICATION TO SEVERAL MEDICINAL HERBS".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 36.15(2013):2107-2124. |
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