Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS

NWIPB OpenIR

	Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS
	Caidan, Rezeng 1,2; Cairang, Limao 3,4; Liubin 1; Suo Yourui 3; Liubin (reprint author), Beijing Univ Chinese Med, Dept Chinese Med Chem, Beijing, Peoples R China.
	2013-04-01
发表期刊	ASIAN JOURNAL OF CHEMISTRY
ISSN	0970-7077
卷号	25 期号:4 页码:1866-1870
文章类型	Article
摘要	The aim of this study was to optimize the microwave extraction conditions of Rubus niveus Thunb. oil from Qinghai-Tibetan plateau via Box-Behnken design of RSM and then analyze the composition of oil by HPLC-MS/MS using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl-4-toluene-sulfonate as labeling reagent. The optimum microwave extraction conditions were as follows: irradiation power 437 W, extraction temperature 75 degrees C and extraction time 12 min. Under these optimized conditions, the maximum oil extraction yield was 5.83 %. All fatty acid derivates had excellent linear responses with correlation coefficients greater than 0.9990. Detection limits (at signal-to-noise of 3:1) were 18.42 to 32.39 pmol. The results indicate that the predominant fatty acids (C10-C22) were 16:0, 18:2, 18:3, 18:0 and 22:0 in total fatty acids. And the unsaturated fatty acids were 18:3, 18:2, 18:1 and 20:4 and reached up to 40.54 % of the total mass contents of fatty acids (C10-C22) in Rubus niveus Thunb. from Qinghai-Tibetan plateau.; The aim of this study was to optimize the microwave extraction conditions of Rubus niveus Thunb. oil from Qinghai-Tibetan plateau via Box-Behnken design of RSM and then analyze the composition of oil by HPLC-MS/MS using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl-4-toluene-sulfonate as labeling reagent. The optimum microwave extraction conditions were as follows: irradiation power 437 W, extraction temperature 75 degrees C and extraction time 12 min. Under these optimized conditions, the maximum oil extraction yield was 5.83 %. All fatty acid derivates had excellent linear responses with correlation coefficients greater than 0.9990. Detection limits (at signal-to-noise of 3:1) were 18.42 to 32.39 pmol. The results indicate that the predominant fatty acids (C10-C22) were 16:0, 18:2, 18:3, 18:0 and 22:0 in total fatty acids. And the unsaturated fatty acids were 18:3, 18:2, 18:1 and 20:4 and reached up to 40.54 % of the total mass contents of fatty acids (C10-C22) in Rubus niveus Thunb. from Qinghai-Tibetan plateau.
关键词	Rubus Niveus Thunb. Fruit Oil Extraction Response Surface Methodology Fatty Acids Hplc-ms
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	LIQUID-CHROMATOGRAPHY ; CARBOXYLIC-ACIDS
收录类别	SCI
语种	英语
项目资助者	Research Fund for self-selected Topic of Beijing University of Chinese Medicine(2011-MZYY-04)
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000312621500025
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3952
专题	中国科学院西北高原生物研究所
通讯作者	Liubin (reprint author), Beijing Univ Chinese Med, Dept Chinese Med Chem, Beijing, Peoples R China.
作者单位	1.Beijing Univ Chinese Med, Dept Chinese Med Chem, Beijing, Peoples R China 2.Qinghai Univ, Coll Med, Res Ctr Chinese & Tibetan Med, Xining, Peoples R China 3.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 4.Qinghai Normal Univ, Coll Nationality Normal, Dept Chem Sci, Xining, Peoples R China
推荐引用方式 GB/T 7714	Caidan, Rezeng,Cairang, Limao,Liubin,et al. Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS[J]. ASIAN JOURNAL OF CHEMISTRY,2013,25(4):1866-1870.
APA	Caidan, Rezeng,Cairang, Limao,Liubin,Suo Yourui,&Liubin .(2013).Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS.ASIAN JOURNAL OF CHEMISTRY,25(4),1866-1870.
MLA	Caidan, Rezeng,et al."Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS".ASIAN JOURNAL OF CHEMISTRY 25.4(2013):1866-1870.