Knowledge Management System of Northwest Institute of Plateau Biology, CAS
High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans | |
Dou, Quan-Wen ; Chen, Zhi-Guo ; Liu, Yong-An ; Tsujimoto, Hisashi | |
2009-12-01 | |
发表期刊 | BREEDING SCIENCE ; Dou, QW; Chen, ZG; Liu, YA; Tsujimoto, H.High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans,BREEDING SCIENCE,2009,59(5):651-656 |
摘要 | Elymus nutans L. (StHY, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the Qinghai-Tibet plateau in China due to its high tolerance to environmental stresses, such as cold and drought. Karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH). GISH discriminated St, H and Y genomes, indicating that E. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. However, FISH using an AGG satellite and Afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. Many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. In addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. The nature of the polymorphisms seen in the chromosomes of the domesticated Population of E. nutans is discussed.; Elymus nutans L. (StHY, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the Qinghai-Tibet plateau in China due to its high tolerance to environmental stresses, such as cold and drought. Karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH). GISH discriminated St, H and Y genomes, indicating that E. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. However, FISH using an AGG satellite and Afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. Many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. In addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. The nature of the polymorphisms seen in the chromosomes of the domesticated Population of E. nutans is discussed. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/40954 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,et al. High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans[J]. BREEDING SCIENCE, Dou, QW; Chen, ZG; Liu, YA; Tsujimoto, H.High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans,BREEDING SCIENCE,2009,59(5):651-656,2009. |
APA | Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,&Tsujimoto, Hisashi.(2009).High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans.BREEDING SCIENCE. |
MLA | Dou, Quan-Wen,et al."High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans".BREEDING SCIENCE (2009). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论