Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi | |
| Jiang, Lei1,2; Tao, Yanduo1; Wang, Dan3; Tang, Chuchen4; Shao, Yun1; Wang, Qilan1; Zhao, Xiaohui1; Zhang, Yaozhou2,5; Mei, Lijuan1 | |
| 2014-11-01 | |
| 发表期刊 | JOURNAL OF SEPARATION SCIENCE
 |
| ISSN | 1615-9306 |
| 卷号 | 37期号:21页码:3060-3066 |
| 文章类型 | Article |
| 摘要 | Animal medicine is an important part in traditional Tibetan medicine. However, information about the chemical composition of animal medicine is very limited, and there is a lack of comprehensive chromatographic purification methods. In the present work, animal medicine Osteon Myospalacem Baileyi was taken as an example and a novel two-dimensional preparative chromatographic method was established for the preparation of single compounds with high purity from the extract of Osteon Myospalacem Baileyi. The first-dimension preparation was carried on a DAISO Silica prep column, and ten fractions were obtained from the 112.3 g crude sample within 12 injections. A diol prep column used in nonaqueous mobile phase was selected for the second-dimension preparation. The purity of the compounds isolated from the crude extract was > 98%, which indicated that the method built in this work was efficient to manufacture single compounds of high purity from the extract of Osteon Myospalacem Baileyi. Additionally, this method showed great potential in the purification of weakly polar chemicals and it could act as a good example in the purification of other traditional animal medicines.; Animal medicine is an important part in traditional Tibetan medicine. However, information about the chemical composition of animal medicine is very limited, and there is a lack of comprehensive chromatographic purification methods. In the present work, animal medicine Osteon Myospalacem Baileyi was taken as an example and a novel two-dimensional preparative chromatographic method was established for the preparation of single compounds with high purity from the extract of Osteon Myospalacem Baileyi. The first-dimension preparation was carried on a DAISO Silica prep column, and ten fractions were obtained from the 112.3 g crude sample within 12 injections. A diol prep column used in nonaqueous mobile phase was selected for the second-dimension preparation. The purity of the compounds isolated from the crude extract was > 98%, which indicated that the method built in this work was efficient to manufacture single compounds of high purity from the extract of Osteon Myospalacem Baileyi. Additionally, this method showed great potential in the purification of weakly polar chemicals and it could act as a good example in the purification of other traditional animal medicines. |
| 关键词 | Diol Prep Column Osteon Myospalacem Baileyi Silica Prep Column Tibetan Medicine Two-dimensional Separation |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; DIOL COLUMN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000344250300006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4177 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China 4.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China 5.Tianjin Int Joint Acad Biomed, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Lei,Tao, Yanduo,Wang, Dan,et al. A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(21):3060-3066. |
| APA | Jiang, Lei.,Tao, Yanduo.,Wang, Dan.,Tang, Chuchen.,Shao, Yun.,...&Mei, Lijuan.(2014).A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi.JOURNAL OF SEPARATION SCIENCE,37(21),3060-3066. |
| MLA | Jiang, Lei,et al."A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi".JOURNAL OF SEPARATION SCIENCE 37.21(2014):3060-3066. |
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