Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry. | |
| Sun, Zhiwei1,2; Wang, Xiaoxiang1,2; Cai, Yiping1,2; Fu, Junqing1,2; You, Jinmao1,2,3 | |
| 2014-03-01 | |
| 发表期刊 | TALANTA
 |
| ISSN | 0039-9140 |
| 卷号 | 120页码:84-93 |
| 文章类型 | Article |
| 摘要 | A new pair of derivatization reagents, d(0)-4-(1-methyl-1H-phenanthro[9,10-dlimidazol-2-yl)phenlamine (d(0)-MPIA) and d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine (d(3)-MPIA) have been designed and synthesized. It was successfully used to label aliphatic aldehydes and the aldehyde derivatives were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The new isotope-coded reagents could easily label aldehydes under acidic conditions in the presence of NaCNBH3. The target derivatives exhibited intense [M+H](+) and regular product ions with electrospray ionization source in positive mode. The d(0)/d(3)-MPIA-aldehydes were monitored by the transitions of [M+H](+) -> m/z 322 and [M+H](+) -> m/z 165, and the obtained detection limits were in the range of 0.18-15.9 pg/mL at signal to noise ratio of 3. The global isotope internal standard technology was employed for quantification analysis with d(3)-MPIA-aldehyde as internal standard for corresponding d(0)-MPIA-aldehyde. Excellent linear responses for relative quantification were observed in the range of 1/10-10/1 with coefficients > 0.998. The developed method has been applied to the quantification of aliphatic aldehydes in selected aquatic products with RSD <3.6% and recoveries > 85.2%. (C) 2013 Elsevier B.V. All rights reserved.; A new pair of derivatization reagents, d(0)-4-(1-methyl-1H-phenanthro[9,10-dlimidazol-2-yl)phenlamine (d(0)-MPIA) and d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine (d(3)-MPIA) have been designed and synthesized. It was successfully used to label aliphatic aldehydes and the aldehyde derivatives were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The new isotope-coded reagents could easily label aldehydes under acidic conditions in the presence of NaCNBH3. The target derivatives exhibited intense [M+H](+) and regular product ions with electrospray ionization source in positive mode. The d(0)/d(3)-MPIA-aldehydes were monitored by the transitions of [M+H](+) -> m/z 322 and [M+H](+) -> m/z 165, and the obtained detection limits were in the range of 0.18-15.9 pg/mL at signal to noise ratio of 3. The global isotope internal standard technology was employed for quantification analysis with d(3)-MPIA-aldehyde as internal standard for corresponding d(0)-MPIA-aldehyde. Excellent linear responses for relative quantification were observed in the range of 1/10-10/1 with coefficients > 0.998. The developed method has been applied to the quantification of aliphatic aldehydes in selected aquatic products with RSD <3.6% and recoveries > 85.2%. (C) 2013 Elsevier B.V. All rights reserved. |
| 关键词 | Isotope-coded Derivatization Reagent Hplc-ms/ms Aliphatic Aldehydes |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 关键词[WOS] | ALIPHATIC-ALDEHYDES ; CARBONYL-COMPOUNDS ; LABELING REAGENT ; OXIDATIVE DAMAGE ; MICROEXTRACTION ; 2,4-DINITROPHENYLHYDRAZINE ; QUANTITATION ; DERIVATIVES ; METABOLITE ; HYDRAZINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000331917700013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4250 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab LifeOrgan Anal Shandong Prov, Qufu, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Zhiwei,Wang, Xiaoxiang,Cai, Yiping,et al. Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry.[J]. TALANTA,2014,120:84-93. |
| APA | Sun, Zhiwei,Wang, Xiaoxiang,Cai, Yiping,Fu, Junqing,&You, Jinmao.(2014).Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry..TALANTA,120,84-93. |
| MLA | Sun, Zhiwei,et al."Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry.".TALANTA 120(2014):84-93. |
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