Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| The microbial gene diversity along an elevation gradient of the Tibetan grassland | |
| Yang, Yunfeng1; Gao, Ying1; Wang, Shiping2; Xu, Depeng1; Yu, Hao3; Wu, Linwei1; Lin, Qiaoyan4; Hu, Yigang4,5; Li, Xiangzhen6; He, Zhili3; Deng, Ye3; Zhou, Jizhong1,3,7 | |
| 2014-02-01 | |
| 发表期刊 | ISME JOURNAL
 |
| ISSN | 1751-7362 |
| 卷号 | 8期号:2页码:430-440 |
| 文章类型 | Article |
| 摘要 | Tibet is one of the most threatened regions by climate warming, thus understanding how its microbial communities function may be of high importance for predicting microbial responses to climate changes. Here, we report a study to profile soil microbial structural genes, which infers functional roles of microbial communities, along four sites/elevations of a Tibetan mountainous grassland, aiming to explore the potential microbial responses to climate changes via a strategy of space-for-time substitution. Using a microarray-based metagenomics tool named GeoChip 4.0, we showed that microbial communities were distinct for most but not all of the sites. Substantial variations were apparent in stress, N and C-cycling genes, but they were in line with the functional roles of these genes. Cold shock genes were more abundant at higher elevations. Also, gdh converting ammonium into urea was more abundant at higher elevations, whereas ureC converting urea into ammonium was less abundant, which was consistent with soil ammonium contents. Significant correlations were observed between N-cycling genes (ureC, gdh and amoA) and nitrous oxide flux, suggesting that they contributed to community metabolism. Lastly, we found by Canonical correspondence analysis, Mantel tests and the similarity tests that soil pH, temperature, NH4+-N and vegetation diversity accounted for the majority (81.4%) of microbial community variations, suggesting that these four attributes were major factors affecting soil microbial communities. On the basis of these observations, we predict that climate changes in the Tibetan grasslands are very likely to change soil microbial community functional structure, with particular impacts on microbial N-cycling genes and consequently microbe-mediated soil N dynamics.; Tibet is one of the most threatened regions by climate warming, thus understanding how its microbial communities function may be of high importance for predicting microbial responses to climate changes. Here, we report a study to profile soil microbial structural genes, which infers functional roles of microbial communities, along four sites/elevations of a Tibetan mountainous grassland, aiming to explore the potential microbial responses to climate changes via a strategy of space-for-time substitution. Using a microarray-based metagenomics tool named GeoChip 4.0, we showed that microbial communities were distinct for most but not all of the sites. Substantial variations were apparent in stress, N and C-cycling genes, but they were in line with the functional roles of these genes. Cold shock genes were more abundant at higher elevations. Also, gdh converting ammonium into urea was more abundant at higher elevations, whereas ureC converting urea into ammonium was less abundant, which was consistent with soil ammonium contents. Significant correlations were observed between N-cycling genes (ureC, gdh and amoA) and nitrous oxide flux, suggesting that they contributed to community metabolism. Lastly, we found by Canonical correspondence analysis, Mantel tests and the similarity tests that soil pH, temperature, NH4+-N and vegetation diversity accounted for the majority (81.4%) of microbial community variations, suggesting that these four attributes were major factors affecting soil microbial communities. On the basis of these observations, we predict that climate changes in the Tibetan grasslands are very likely to change soil microbial community functional structure, with particular impacts on microbial N-cycling genes and consequently microbe-mediated soil N dynamics. |
| 关键词 | Gene Diversity Soil Microbial Community Community Metabolism Alpine Grassland Elevation Gradient |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | ECOSYSTEM CO2 EXCHANGE ; GEOCHIP-BASED ANALYSIS ; ALPINE MEADOW ; BACTERIAL COMMUNITIES ; SOIL-MOISTURE ; PLATEAU ; SEA ; PATTERNS ; NITROGEN ; DESERT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Environmental Sciences & Ecology ; Microbiology |
| WOS类目 | Ecology ; Microbiology |
| WOS记录号 | WOS:000330386500016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4258 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Inst Tibetan Plateau Res, Lab Alpine Ecol & Biodivers, Beijing, Peoples R China 3.Univ Oklahoma, Dept Bot & Microbiol, Inst Environm Genom, Norman, OK 73019 USA 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adapt & Evolut Plateau Biota, Xining, Peoples R China 5.Chinese Acad Sci, Cold & Arid Reg & Environm & Engn Res Inst, Shapotou Desert Expt & Res Stn, Lanzhou, Peoples R China 6.Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Peoples R China 7.Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA |
| 推荐引用方式 GB/T 7714 | Yang, Yunfeng,Gao, Ying,Wang, Shiping,et al. The microbial gene diversity along an elevation gradient of the Tibetan grassland[J]. ISME JOURNAL,2014,8(2):430-440. |
| APA | Yang, Yunfeng.,Gao, Ying.,Wang, Shiping.,Xu, Depeng.,Yu, Hao.,...&Zhou, Jizhong.(2014).The microbial gene diversity along an elevation gradient of the Tibetan grassland.ISME JOURNAL,8(2),430-440. |
| MLA | Yang, Yunfeng,et al."The microbial gene diversity along an elevation gradient of the Tibetan grassland".ISME JOURNAL 8.2(2014):430-440. |
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