Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography

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	Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography
	He, Yanfeng 1,2; Lv, Huanhuan 1,2; Wang, Xiaoyan 1; Suo, Yourui 1; Wang, Honglun 1
	2014-01-31
发表期刊	SEPARATION SCIENCE AND TECHNOLOGY
ISSN	0149-6395
卷号	49 期号:4 页码:580-587
文章类型	Article
摘要	It is difficult to simultaneously isolate and purify flavonoids and polyhydroxystilbenes due to their similar polarities. In this study, we isolated and purified these compounds from Trigonella foenum-graecum L. seed extracts using high-speed counter-current chromatography (HSCCC) in two steps with two different kinds of solvent systems. In the first step, n-hexane:ethyl acetate:methanol:water (1:5:1:5, v/v) was used as the two-phase solvent system, desoxy-rhaponticin and rhapontigenin were purified, and orientin, vitexin, isovitexin, and rhaponticin were eluted together. In the second step, ethyl acetate:water (1:1, v/v) was used as the two-phase solvent system, and orientin, vitexin, rhaponticin, and isovitexin were isolated and purified. Purities of the isolated compounds were determined using HPLC, while UV spectra, H- 1 -NMR, and C- 13 -NMR aided in structure identification.; It is difficult to simultaneously isolate and purify flavonoids and polyhydroxystilbenes due to their similar polarities. In this study, we isolated and purified these compounds from Trigonella foenum-graecum L. seed extracts using high-speed counter-current chromatography (HSCCC) in two steps with two different kinds of solvent systems. In the first step, n-hexane:ethyl acetate:methanol:water (1:5:1:5, v/v) was used as the two-phase solvent system, desoxy-rhaponticin and rhapontigenin were purified, and orientin, vitexin, isovitexin, and rhaponticin were eluted together. In the second step, ethyl acetate:water (1:1, v/v) was used as the two-phase solvent system, and orientin, vitexin, rhaponticin, and isovitexin were isolated and purified. Purities of the isolated compounds were determined using HPLC, while UV spectra, H- 1 -NMR, and C- 13 -NMR aided in structure identification.
关键词	Flavonoids High-speed Counter-current Chromatography Trigonella Foenum-graecum l Preparative Isolation Polyhydroxystilbenes
WOS标题词	Science & Technology ; Physical Sciences ; Technology
关键词[WOS]	PREPARATIVE SEPARATION ; ANTIOXIDANT ACTIVITIES ; SOLVENT SYSTEMS ; FENUGREEK SEEDS ; RHEUM-UNDULATUM ; RHAPONTIGENIN ; EXTRACT ; DAMAGE ; MICE
收录类别	SCI
语种	英语
WOS研究方向	Chemistry ; Engineering
WOS类目	Chemistry, Multidisciplinary ; Engineering, Chemical
WOS记录号	WOS:000331526700012
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/4261
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	He, Yanfeng,Lv, Huanhuan,Wang, Xiaoyan,et al. Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2014,49(4):580-587.
APA	He, Yanfeng,Lv, Huanhuan,Wang, Xiaoyan,Suo, Yourui,&Wang, Honglun.(2014).Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography.SEPARATION SCIENCE AND TECHNOLOGY,49(4),580-587.
MLA	He, Yanfeng,et al."Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography".SEPARATION SCIENCE AND TECHNOLOGY 49.4(2014):580-587.