Genetic variation in cultivated Rheum tanguticum populations

NWIPB OpenIR

	Genetic variation in cultivated Rheum tanguticum populations
	Hu, Yanping 1; Xie, Xiaolong 2; Wang, Li 1; Zhang, Huaigang 1; Yang, Jian 1; Li, Yi 1
	2014
发表期刊	GENETICS AND MOLECULAR BIOLOGY
ISSN	1415-4757
卷号	37 期号:3 页码:540-548
文章类型	Article
摘要	To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, H-B = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient of gene differentiation (G(st) = 0.2742) and Bayesian analysis (theta(B) = 0.1963). The Mantel test revealed no significant correlation between genetic and geographic distances among populations (r = 0.1176, p = 0.3686). UPGMA showed that the five cultivated populations were separated into three clusters, which was in good accordance with the results provided by the Bayesian software STRUCTURE (K = 3). A short domestication history and no artificial selection may be an effective way of maintaining and conserving the gene pools of wild R. tanguticum.; To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, H-B = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient of gene differentiation (G(st) = 0.2742) and Bayesian analysis (theta(B) = 0.1963). The Mantel test revealed no significant correlation between genetic and geographic distances among populations (r = 0.1176, p = 0.3686). UPGMA showed that the five cultivated populations were separated into three clusters, which was in good accordance with the results provided by the Bayesian software STRUCTURE (K = 3). A short domestication history and no artificial selection may be an effective way of maintaining and conserving the gene pools of wild R. tanguticum.
关键词	Cultivated Populations Genetic Variation Issr Polygonaceae Rheum Tanguticum Maxim. Ex Balf
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	MULTILOCUS GENOTYPE DATA ; QINGHAI-TIBET PLATEAU ; ISSR MARKERS ; DIVERSITY ; WILD ; CHINA ; RHUBARB ; L. ; DOMESTICATION ; POLYGONACEAE
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:000342995600010
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/4267
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China 2.Henan Univ Tradit Chinese Med, Sch Pharm, Zhengzhou, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yanping,Xie, Xiaolong,Wang, Li,et al. Genetic variation in cultivated Rheum tanguticum populations[J]. GENETICS AND MOLECULAR BIOLOGY,2014,37(3):540-548.
APA	Hu, Yanping,Xie, Xiaolong,Wang, Li,Zhang, Huaigang,Yang, Jian,&Li, Yi.(2014).Genetic variation in cultivated Rheum tanguticum populations.GENETICS AND MOLECULAR BIOLOGY,37(3),540-548.
MLA	Hu, Yanping,et al."Genetic variation in cultivated Rheum tanguticum populations".GENETICS AND MOLECULAR BIOLOGY 37.3(2014):540-548.

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