Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum | |
| Chen, Tao1; Liu, Yongling1,2; Zou, Denglang1,2; Chen, Chen1; You, Jinmao1; Zhou, Guoying1; Sun, Jing1; Li, Yulin1 | |
| 2014 | |
| 发表期刊 | JOURNAL OF SEPARATION SCIENCE
 |
| ISSN | 1615-9306 |
| 卷号 | 37期号:1-2页码:165-170 |
| 文章类型 | Article |
| 摘要 | This study presents an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum. A new solvent system composed of petroleum ether/ethyl acetate/water (4:2:1, v/v/v) was developed for the liquid-liquid extraction of the crude extract from R. tanguticum. As a result, emodin, aloe-emodin, physcion, and chrysophanol were greatly enriched in the organic layer. In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. This study supplies a new alternative method for the rapid enrichment, separation, and purification of emodin, aloe-emodin, physcione, and chrysophanol.; This study presents an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum. A new solvent system composed of petroleum ether/ethyl acetate/water (4:2:1, v/v/v) was developed for the liquid-liquid extraction of the crude extract from R. tanguticum. As a result, emodin, aloe-emodin, physcion, and chrysophanol were greatly enriched in the organic layer. In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. This study supplies a new alternative method for the rapid enrichment, separation, and purification of emodin, aloe-emodin, physcione, and chrysophanol. |
| 关键词 | Anthraquinones High-speed Counter-current Chromatography Liquid-liquid Extraction Preparative Hplc Rheum Tanguticum |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 关键词[WOS] | STILBENE GLYCOSIDES ; CONSTITUENTS ; PERFORMANCE ; CCC |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000329478100024 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4292 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Tao,Liu, Yongling,Zou, Denglang,et al. Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(1-2):165-170. |
| APA | Chen, Tao.,Liu, Yongling.,Zou, Denglang.,Chen, Chen.,You, Jinmao.,...&Li, Yulin.(2014).Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum.JOURNAL OF SEPARATION SCIENCE,37(1-2),165-170. |
| MLA | Chen, Tao,et al."Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum".JOURNAL OF SEPARATION SCIENCE 37.1-2(2014):165-170. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Application of an ef（516KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论