A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi

NWIPB OpenIR

	A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi
	Jiang, Lei ; Tao, Yanduo ; Wang, Dan ; Tang, Chuchen ; Shao, Yun ; Wang, Qilan ; Zhao, Xiaohui ; Zhang, Yaozhou ; Mei, Lijuan
	2014-11-01
发表期刊	JOURNAL OF SEPARATION SCIENCE ; Jiang, L; Tao, YD; Wang, D; Tang, CC; Shao, Y; Wang, QL; Zhao, XH; Zhang, YZ; Mei, LJ.A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi,JOURNAL OF SEPARATION SCIENCE,2014,37(21):3060
摘要	Animal medicine is an important part in traditional Tibetan medicine. However, information about the chemical composition of animal medicine is very limited, and there is a lack of comprehensive chromatographic purification methods. In the present work, animal medicine Osteon Myospalacem Baileyi was taken as an example and a novel two-dimensional preparative chromatographic method was established for the preparation of single compounds with high purity from the extract of Osteon Myospalacem Baileyi. The first-dimension preparation was carried on a DAISO Silica prep column, and ten fractions were obtained from the 112.3 g crude sample within 12 injections. A diol prep column used in nonaqueous mobile phase was selected for the second-dimension preparation. The purity of the compounds isolated from the crude extract was > 98%, which indicated that the method built in this work was efficient to manufacture single compounds of high purity from the extract of Osteon Myospalacem Baileyi. Additionally, this method showed great potential in the purification of weakly polar chemicals and it could act as a good example in the purification of other traditional animal medicines.; Animal medicine is an important part in traditional Tibetan medicine. However, information about the chemical composition of animal medicine is very limited, and there is a lack of comprehensive chromatographic purification methods. In the present work, animal medicine Osteon Myospalacem Baileyi was taken as an example and a novel two-dimensional preparative chromatographic method was established for the preparation of single compounds with high purity from the extract of Osteon Myospalacem Baileyi. The first-dimension preparation was carried on a DAISO Silica prep column, and ten fractions were obtained from the 112.3 g crude sample within 12 injections. A diol prep column used in nonaqueous mobile phase was selected for the second-dimension preparation. The purity of the compounds isolated from the crude extract was > 98%, which indicated that the method built in this work was efficient to manufacture single compounds of high purity from the extract of Osteon Myospalacem Baileyi. Additionally, this method showed great potential in the purification of weakly polar chemicals and it could act as a good example in the purification of other traditional animal medicines.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/47505
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jiang, Lei,Tao, Yanduo,Wang, Dan,et al. A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi[J]. JOURNAL OF SEPARATION SCIENCE, Jiang, L; Tao, YD; Wang, D; Tang, CC; Shao, Y; Wang, QL; Zhao, XH; Zhang, YZ; Mei, LJ.A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi,JOURNAL OF SEPARATION SCIENCE,2014,37(21):3060,2014.
APA	Jiang, Lei.,Tao, Yanduo.,Wang, Dan.,Tang, Chuchen.,Shao, Yun.,...&Mei, Lijuan.(2014).A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi.JOURNAL OF SEPARATION SCIENCE.
MLA	Jiang, Lei,et al."A novel two-dimensional preparative chromatography method designed for the separation of traditional animal Tibetan medicine Osteon Myospalacem Baileyi".JOURNAL OF SEPARATION SCIENCE (2014).