Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum

NWIPB OpenIR

	Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum
	Chen, Tao ; Liu, Yongling ; Zou, Denglang ; Chen, Chen ; You, Jinmao ; Zhou, Guoying ; Sun, Jing ; Li, Yulin
	2014
发表期刊	JOURNAL OF SEPARATION SCIENCE ; Chen, T; Liu, YL; Zou, DL; Chen, C; You, JM; Zhou, GY; Sun, J; Li, YL.Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum,JOURNAL OF SEPARATION SCIENCE,2014,37(41641):165
摘要	This study presents an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum. A new solvent system composed of petroleum ether/ethyl acetate/water (4:2:1, v/v/v) was developed for the liquid-liquid extraction of the crude extract from R. tanguticum. As a result, emodin, aloe-emodin, physcion, and chrysophanol were greatly enriched in the organic layer. In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. This study supplies a new alternative method for the rapid enrichment, separation, and purification of emodin, aloe-emodin, physcione, and chrysophanol.; This study presents an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum. A new solvent system composed of petroleum ether/ethyl acetate/water (4:2:1, v/v/v) was developed for the liquid-liquid extraction of the crude extract from R. tanguticum. As a result, emodin, aloe-emodin, physcion, and chrysophanol were greatly enriched in the organic layer. In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. This study supplies a new alternative method for the rapid enrichment, separation, and purification of emodin, aloe-emodin, physcione, and chrysophanol.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/47620
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Tao,Liu, Yongling,Zou, Denglang,et al. Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum[J]. JOURNAL OF SEPARATION SCIENCE, Chen, T; Liu, YL; Zou, DL; Chen, C; You, JM; Zhou, GY; Sun, J; Li, YL.Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum,JOURNAL OF SEPARATION SCIENCE,2014,37(41641):165,2014.
APA	Chen, Tao.,Liu, Yongling.,Zou, Denglang.,Chen, Chen.,You, Jinmao.,...&Li, Yulin.(2014).Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum.JOURNAL OF SEPARATION SCIENCE.
MLA	Chen, Tao,et al."Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum".JOURNAL OF SEPARATION SCIENCE (2014).