Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC

NWIPB OpenIR

	Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC
	Zhang, Lin ; Yue, Hui-Lan ; Zhao, Xiao-Hui ; Li, Jing ; Shao, Yun
	2015-07-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
摘要	Four phenylpropanoid glycosides (PPGs), such as echinacoside, wiede-mannioside C, forsythoside B and verbascoside, were isolated and purified from the Tibetan medicinal herb Pedicularis longiflora Rudolph. var. tubiformis (Klotz) Tsoong by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation for the first time. In the present study, the two-phase solvent system composed of ethyl acetate-n-butanol-water (10 : 6: 15, v/v/v) was used for HSCCC separation. A total of 3.5 mg of echinacoside, 12.6 mg of wiede-mannioside C, 22.7 mg of forsythoside B and 48.7 mg of verbascoside with the purity of 93.6, 97.9, 97.8 and 98.1%, respectively, were obtained from 120 mg of crude sample. The HSCCC fractions were analyzed by HPLC, and the chemical structures were identified by H-1 NMR and C-13 NMR. The results demonstrate that MR coupled with HSCCC is a powerful technique for separation of PPGs from natural products.; Four phenylpropanoid glycosides (PPGs), such as echinacoside, wiede-mannioside C, forsythoside B and verbascoside, were isolated and purified from the Tibetan medicinal herb Pedicularis longiflora Rudolph. var. tubiformis (Klotz) Tsoong by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation for the first time. In the present study, the two-phase solvent system composed of ethyl acetate-n-butanol-water (10 : 6: 15, v/v/v) was used for HSCCC separation. A total of 3.5 mg of echinacoside, 12.6 mg of wiede-mannioside C, 22.7 mg of forsythoside B and 48.7 mg of verbascoside with the purity of 93.6, 97.9, 97.8 and 98.1%, respectively, were obtained from 120 mg of crude sample. The HSCCC fractions were analyzed by HPLC, and the chemical structures were identified by H-1 NMR and C-13 NMR. The results demonstrate that MR coupled with HSCCC is a powerful technique for separation of PPGs from natural products.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/47879
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Lin,Yue, Hui-Lan,Zhao, Xiao-Hui,et al. Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015.
APA	Zhang, Lin,Yue, Hui-Lan,Zhao, Xiao-Hui,Li, Jing,&Shao, Yun.(2015).Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA	Zhang, Lin,et al."Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2015).