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HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L | |
Song, Cuihua ; Sun, Zhiwei ; Xia, Lian ; Suo, Yourui ; You, Jinmao | |
2010 | |
发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES ; Song, CH; Sun, ZW; Xia, L; Suo, YR; You, JM.HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(6):859-874 |
摘要 | A sensitive HPLC method for the determination of fatty acids using 2-(2-(pyren-1-yl)-1H-benzo[d]imidazol-1-yl) ethyl-p-toluenesulfonate (PBIOTs) as a novel labeling reagent with fluorescence detection has been developed. PBIOTs could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 30min in N,N-dimethylformamide solvent. Fatty acids derivatives were separated on a reversed phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI-MS in positive ion detection mode. PBIOTs-fatty acid derivatives gave an intense molecular ion peak at m/z [M+H]+; with MS/MS analysis, the collision induced dissociation spectra of m/z [M+H]+ produced the specific fragment ions at m/z [M+H-319]+ and m/z 319.0 (here, m/z 319 is the core structural moiety of the PBIOTs molecule). The fluorescence excitation and emission wavelengths of the derivatives were ex=350nm and em=402nm, respectively. Linear correlation coefficients for all fatty acid derivatives were epsilon 0.9985. Detection limits for the labeled fatty acids, at a signal-to-noise ratio of 3:1, were in the range of 10.32- 44.28fmol. From the extracts of Stellera chamaejasma L, free fatty acids were sensitively determined.; A sensitive HPLC method for the determination of fatty acids using 2-(2-(pyren-1-yl)-1H-benzo[d]imidazol-1-yl) ethyl-p-toluenesulfonate (PBIOTs) as a novel labeling reagent with fluorescence detection has been developed. PBIOTs could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 30min in N,N-dimethylformamide solvent. Fatty acids derivatives were separated on a reversed phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI-MS in positive ion detection mode. PBIOTs-fatty acid derivatives gave an intense molecular ion peak at m/z [M+H]+; with MS/MS analysis, the collision induced dissociation spectra of m/z [M+H]+ produced the specific fragment ions at m/z [M+H-319]+ and m/z 319.0 (here, m/z 319 is the core structural moiety of the PBIOTs molecule). The fluorescence excitation and emission wavelengths of the derivatives were ex=350nm and em=402nm, respectively. Linear correlation coefficients for all fatty acid derivatives were epsilon 0.9985. Detection limits for the labeled fatty acids, at a signal-to-noise ratio of 3:1, were in the range of 10.32- 44.28fmol. From the extracts of Stellera chamaejasma L, free fatty acids were sensitively determined. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/51111 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Song, Cuihua,Sun, Zhiwei,Xia, Lian,et al. HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, Song, CH; Sun, ZW; Xia, L; Suo, YR; You, JM.HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(6):859-874,2010. |
APA | Song, Cuihua,Sun, Zhiwei,Xia, Lian,Suo, Yourui,&You, Jinmao.(2010).HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES. |
MLA | Song, Cuihua,et al."HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2010). |
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