Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology | |
Cui, Yulei ; Shen, Na ; Dang, Jun ; Mei, Lijuan ; Tao, Yanduo ; Liu, Zenggen | |
2017-07-01 | |
发表期刊 | JOURNAL OF SEPARATION SCIENCE |
摘要 | Bioactive equivalent combinatorial components play a critical role in herbal medicines. However, how to discover and enrich them efficiently is a question for herbal pharmaceuticals researchers. In our work, a novel two-dimensional reversed-phase/hydrophilic interaction high-performance liquid chromatography method was established to perform real-time components trapping and combining for preparation and isolation of coeluting components. Arenaria kansuensis was taken as an example, and solid-phase extraction coupled with liquid-liquid extraction as a simple and efficient method for enriching trace components, reversed phase column coupled with hydrophilic interaction liquid chromatography XAmide column as two-dimensional chromatography technology for isolation and preparation of coeluting constituents, enzyme-linked immune-sorbent assay as bio-guided assay, and anti-inflammatory bioactivity evaluation for bioactive constituents. A combination of 12 beta-carboline alkaloidswas identified as anti-inflammatory bioactive equivalent combinatorial components from A. kansuensis, which accounts for 1.9% w/w of original A. kansuensis. This work answers the key question of which are real anti-inflammatory components from A. kansuensis and provides a fast and efficient approach for discovering and enriching trace beta-carboline alkaloids from herbal medicines for the first time. More importantly, the discovery of bioactive equivalent combinatorial components could improve the quality control of herbal products and inspire a herbal medicine based on combinatorial therapeutics. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/53720 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Cui, Yulei,Shen, Na,Dang, Jun,et al. Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology[J]. JOURNAL OF SEPARATION SCIENCE,2017. |
APA | Cui, Yulei,Shen, Na,Dang, Jun,Mei, Lijuan,Tao, Yanduo,&Liu, Zenggen.(2017).Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology.JOURNAL OF SEPARATION SCIENCE. |
MLA | Cui, Yulei,et al."Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology".JOURNAL OF SEPARATION SCIENCE (2017). |
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