Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway

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	Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway
	Zhao, Xiaoying ; Mei, Lijuan ; Pei, Jinjin ; Liu, Zenggen ; Shao, Yun ; Tao, Yanduo ; Zhang, Xiaoling ; Jiang, Lei
	2017-11-08
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
摘要	An imbalance in osteogenesis and osteoclastogenesis is a crucial pathological factor in the development of osteoporosis. Osteoclasts (OCs) play a pivotal role in osteoporosis, whose new therapy exploration has been focused on the suppression of OC formation. Sophoridine is found from the Chinese traditional food sophora flower to exhibit anti-osteoporosis capacity by screening. This study is focused on its anti -osteoporosis mechanism evaluation. The anti -osteoporosis effect of sophoridine, (15 mg kg(-1) body), was evaluated in ovariectomized (OVX) mice by monitoring changes in bone histomorphometry index, formation of osteoclasts from blood-derived mononuclear cells, and changes in the synthesis of pro-osteodastogenic cytokines. Signal pathways were investigated by QPCR, Western blot, and immunofluorescence. Sophoridine has a significant anti-osteoporosis effect in vivo, which can inhibit RANKL-induced OC formation, the appearance of OC-specific marker genes, and OC marker protein in vitro. Mechanistically, sophoridine dose- and time-dependently blocks the RANKL-induced OC formation and the activation of ERK and c-Fos as well as the induction and nucleus translocation of NFATc1. Sophora flower might be a useful alternative functional food in preventing or treating osteoporosis.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/54275
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, Xiaoying,Mei, Lijuan,Pei, Jinjin,et al. Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017.
APA	Zhao, Xiaoying.,Mei, Lijuan.,Pei, Jinjin.,Liu, Zenggen.,Shao, Yun.,...&Jiang, Lei.(2017).Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.
MLA	Zhao, Xiaoying,et al."Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2017).