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| Luteolin inhibits ROS-activated MAPK pathway in myocardial ischemia/reperfusion injury | |
| Yu, Dongsheng1,2; Li, Mengwen1,2; Tian, Youqing3; Liu, Jun1,2; Shang, Jing1,2,4; Shang, J (reprint author), China Pharmaceut Univ, Ctr Drug Screening, 24 Tong Jia Xiang, Nanjing 210009, Jiangsu, Peoples R China. | |
| 2015-02-01 | |
| 发表期刊 | LIFE SCIENCES
 |
| 卷号 | 122页码:15-25 |
| 文章类型 | Article |
| 摘要 | Aims: Luteolin is a falconoid compound that has an antioxidant effect, but its contribution to ROS-activated MAPK pathways in ischemia/reperfusion injury is seldom reported. Here, we have confirmed that it exhibits an antioxidant effect in myocardial ischemia/reperfusion injury (MIRI) by inhibiting ROS-activated MAPK pathways. Main methods: We exposed rat hearts into the left anterior descending coronary artery (LAD) ligation for 30 min followed by 1 h of reperfusion. Observations were carried out using electrocardiography; detection of hemodynamic parameters; and testing levels of lactate dehydrogenase (LDH), creatine kinase (CK), total superoxide dismutase (T-SOD), and malondialdehyde (MDA). Mitogen-activated protein kinase (MAPK) pathway was measured by western blot and transmission electron microscopy was applied to observe the myocardial ultrastructure. Rat H9c2 cell in 95% N-2 and 5% CO2 stimulated the MIRI. Oxidation system mRNA levels were measured by real-time PCR; mitochondrial membrane potential and apoptosis were measured by confocal microscopy and flow cytometry; western blot analysis was used to assay caspase-3, -8, and -9 and MAPK pathway protein expression; the MAPK pathway was inhibited using SB203580 (p38 MAPK inhibitor) and SP600125 (c-Jun NH2-terminal kinase inhibitor) before H9c2 cells were exposed to hypoxia/reoxygenation injury to show the modulation of the changes in ROS generation, cell viability and apoptosis. Key findings: In vivo, luteolin can ameliorate the impaired mitochondrial morphology, regulating the MAPK pathway to protect MIRI. In vitro, luteolin can affect the oxidation system, mitochondrial membrane potential and MAPK pathway to anti-apoptosis. Significance: These results reveal a ROS-MAPK mediated mechanism and mitochondrial pathway through which luteolin can protect myocardial ischemia/reperfusion injury. (C) 2014 Elsevier Inc. All rights reserved.; Aims: Luteolin is a falconoid compound that has an antioxidant effect, but its contribution to ROS-activated MAPK pathways in ischemia/reperfusion injury is seldom reported. Here, we have confirmed that it exhibits an antioxidant effect in myocardial ischemia/reperfusion injury (MIRI) by inhibiting ROS-activated MAPK pathways. |
| 关键词 | Luteolin Myocardial Ischemia/reperfusion Injury Reactive Oxygen Species Mapk Mitochondrial Apoptosis |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | ISCHEMIA-REPERFUSION INJURY ; UP-REGULATION ; INFARCT SIZE ; IN-VITRO ; APOPTOSIS ; CELLS ; PROTECTS ; HYPOXIA/REOXYGENATION ; KINASE ; HEART |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| WOS类目 | Medicine, Research & Experimental ; Pharmacology & Pharmacy |
| WOS记录号 | WOS:000349508500003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/5458 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Shang, J (reprint author), China Pharmaceut Univ, Ctr Drug Screening, 24 Tong Jia Xiang, Nanjing 210009, Jiangsu, Peoples R China. |
| 作者单位 | 1.China Pharmaceut Univ, Ctr Drug Screening, Nanjing 210009, Jiangsu, Peoples R China 2.China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Jiangsu, Peoples R China 3.Jiangsu Union Tech Inst, Lianyungang TCM Branch, Lianyungang 222007, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, Xining 810008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Dongsheng,Li, Mengwen,Tian, Youqing,et al. Luteolin inhibits ROS-activated MAPK pathway in myocardial ischemia/reperfusion injury[J]. LIFE SCIENCES,2015,122:15-25. |
| APA | Yu, Dongsheng,Li, Mengwen,Tian, Youqing,Liu, Jun,Shang, Jing,&Shang, J .(2015).Luteolin inhibits ROS-activated MAPK pathway in myocardial ischemia/reperfusion injury.LIFE SCIENCES,122,15-25. |
| MLA | Yu, Dongsheng,et al."Luteolin inhibits ROS-activated MAPK pathway in myocardial ischemia/reperfusion injury".LIFE SCIENCES 122(2015):15-25. |
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| Yu-2015-Luteolin inh（1649KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
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