Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity

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	Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity
	Chen, Chen; Shao, Yun; Tao, Yanduo; Wen, Huaixiu
	2015-12-01
发表期刊	LWT-FOOD SCIENCE AND TECHNOLOGY
卷号	64 期号:2 页码:1263-1269
文章类型	Article
摘要	We used the Box Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X-1), microwave power (X-2) and water to raw material ratio (X-3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X-1 X-3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction, The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X-1 = 30.24 min, X-2 = 600.6 W and X-3 = 40 mL/g. The model was verified by modifying the optimal conditions (X-1 = 30 min, X-2 = 601 Wand X-3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 +/- 0.12% and 8.34 +/- 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI-H446 cells. (C) 2015 Elsevier Ltd. All rights reserved.; We used the Box Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X-1), microwave power (X-2) and water to raw material ratio (X-3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X-1 X-3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction, The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X-1 = 30.24 min, X-2 = 600.6 W and X-3 = 40 mL/g. The model was verified by modifying the optimal conditions (X-1 = 30 min, X-2 = 601 Wand X-3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 +/- 0.12% and 8.34 +/- 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI-H446 cells. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Armillaria Luteo-virens (Alb. Et Schw. Fr.) Sacc Polysaccharide Dynamic Microwave-assisted Extraction Response Surface Methodology Biological Activity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	RADICAL ABSORBENCY CAPACITY ; CRUDE POLYSACCHARIDES ; ANTIOXIDANT ACTIVITY ; ASSAY
收录类别	SCI
语种	英语
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:000360773500105
引用统计	被引频次：72[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5489
专题	中国科学院西北高原生物研究所
作者单位	Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Chen, Chen,Shao, Yun,Tao, Yanduo,et al. Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity[J]. LWT-FOOD SCIENCE AND TECHNOLOGY,2015,64(2):1263-1269.
APA	Chen, Chen,Shao, Yun,Tao, Yanduo,&Wen, Huaixiu.(2015).Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity.LWT-FOOD SCIENCE AND TECHNOLOGY,64(2),1263-1269.
MLA	Chen, Chen,et al."Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity".LWT-FOOD SCIENCE AND TECHNOLOGY 64.2(2015):1263-1269.

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