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Sensitive Determination of Taurine, gamma-Aminobutyric Acid and Ornithine in Wolfberry Fruit and Cortex Lycii by HPLC with Fluorescence Detection and Online Mass Spectrometry Identification
Chen, Xiangming1,2,3; You, Jinmao1,4; Suo, Yourui1; Fan, Baolei1,3
2015-04-01
发表期刊JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷号53期号:4页码:492-497
文章类型Article
摘要A new, simple and highly sensitive method for the determination of taurine, gamma-aminobutyric acid and ornithine using high-performance liquid chromatography (HPLC) with fluorescence detection is described. Three non-protein amino acids were derivatized by a novel precolumn derivatization reagent 2-[2-(dibenzocarbazol)-ethoxy]ethyl chloroformate before injected. Optimum derivatization was obtained at 40 degrees C for 5 min in the presence of sodium borate buffer (pH 9.0). Derivatives were sufficiently stable to be efficiently analyzed by HPLC without pretreatment. On a reversed-phase Hypersil BDS C8 column, the amino acids were separated in conjunction with a gradient elution with a good baseline resolution. The identification of derivatives was carried out by online postcolumn mass spectrometry with an electrospray ionization source in positive ion mode. Excellent linear responses were observed with the correlation coefficients of >0.9996, and instrument detection limits (at a signal to noise of 3 : 1) were in the range of 0.30-0.33 nmol/L. The proposed method is sensitive and reproducible for the precise determination of the amino acids from wolfberry fruit and cortex lycii samples.; A new, simple and highly sensitive method for the determination of taurine, gamma-aminobutyric acid and ornithine using high-performance liquid chromatography (HPLC) with fluorescence detection is described. Three non-protein amino acids were derivatized by a novel precolumn derivatization reagent 2-[2-(dibenzocarbazol)-ethoxy]ethyl chloroformate before injected. Optimum derivatization was obtained at 40 degrees C for 5 min in the presence of sodium borate buffer (pH 9.0). Derivatives were sufficiently stable to be efficiently analyzed by HPLC without pretreatment. On a reversed-phase Hypersil BDS C8 column, the amino acids were separated in conjunction with a gradient elution with a good baseline resolution. The identification of derivatives was carried out by online postcolumn mass spectrometry with an electrospray ionization source in positive ion mode. Excellent linear responses were observed with the correlation coefficients of >0.9996, and instrument detection limits (at a signal to noise of 3 : 1) were in the range of 0.30-0.33 nmol/L. The proposed method is sensitive and reproducible for the precise determination of the amino acids from wolfberry fruit and cortex lycii samples.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; AMINO-ACIDS ; HUMAN PLASMA ; BIOGENIC-AMINES ; REAGENT ; DERIVATIZATION ; CHLOROFORMATE ; GABA ; GLUTAMATE ; SAMPLES
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000356593600007
引用统计
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/5566
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China
2.Binzhou Med Univ, Coll Pharm, Yantai, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China
推荐引用方式
GB/T 7714
Chen, Xiangming,You, Jinmao,Suo, Yourui,et al. Sensitive Determination of Taurine, gamma-Aminobutyric Acid and Ornithine in Wolfberry Fruit and Cortex Lycii by HPLC with Fluorescence Detection and Online Mass Spectrometry Identification[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(4):492-497.
APA Chen, Xiangming,You, Jinmao,Suo, Yourui,&Fan, Baolei.(2015).Sensitive Determination of Taurine, gamma-Aminobutyric Acid and Ornithine in Wolfberry Fruit and Cortex Lycii by HPLC with Fluorescence Detection and Online Mass Spectrometry Identification.JOURNAL OF CHROMATOGRAPHIC SCIENCE,53(4),492-497.
MLA Chen, Xiangming,et al."Sensitive Determination of Taurine, gamma-Aminobutyric Acid and Ornithine in Wolfberry Fruit and Cortex Lycii by HPLC with Fluorescence Detection and Online Mass Spectrometry Identification".JOURNAL OF CHROMATOGRAPHIC SCIENCE 53.4(2015):492-497.
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