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题名: Sensitive Determination of Taurine, gamma-Aminobutyric Acid and Ornithine in Wolfberry Fruit and Cortex Lycii by HPLC with Fluorescence Detection and Online Mass Spectrometry Identification
作者: Chen, Xiangming1, 2, 3; You, Jinmao1, 4; Suo, Yourui1; Fan, Baolei1, 3
刊名: JOURNAL OF CHROMATOGRAPHIC SCIENCE
出版日期: 2015-04-01
卷号: 53, 期号:4, 页码:492-497
文章类型: Article
中文摘要: A new, simple and highly sensitive method for the determination of taurine, gamma-aminobutyric acid and ornithine using high-performance liquid chromatography (HPLC) with fluorescence detection is described. Three non-protein amino acids were derivatized by a novel precolumn derivatization reagent 2-[2-(dibenzocarbazol)-ethoxy]ethyl chloroformate before injected. Optimum derivatization was obtained at 40 degrees C for 5 min in the presence of sodium borate buffer (pH 9.0). Derivatives were sufficiently stable to be efficiently analyzed by HPLC without pretreatment. On a reversed-phase Hypersil BDS C8 column, the amino acids were separated in conjunction with a gradient elution with a good baseline resolution. The identification of derivatives was carried out by online postcolumn mass spectrometry with an electrospray ionization source in positive ion mode. Excellent linear responses were observed with the correlation coefficients of >0.9996, and instrument detection limits (at a signal to noise of 3 : 1) were in the range of 0.30-0.33 nmol/L. The proposed method is sensitive and reproducible for the precise determination of the amino acids from wolfberry fruit and cortex lycii samples.
英文摘要: A new, simple and highly sensitive method for the determination of taurine, gamma-aminobutyric acid and ornithine using high-performance liquid chromatography (HPLC) with fluorescence detection is described. Three non-protein amino acids were derivatized by a novel precolumn derivatization reagent 2-[2-(dibenzocarbazol)-ethoxy]ethyl chloroformate before injected. Optimum derivatization was obtained at 40 degrees C for 5 min in the presence of sodium borate buffer (pH 9.0). Derivatives were sufficiently stable to be efficiently analyzed by HPLC without pretreatment. On a reversed-phase Hypersil BDS C8 column, the amino acids were separated in conjunction with a gradient elution with a good baseline resolution. The identification of derivatives was carried out by online postcolumn mass spectrometry with an electrospray ionization source in positive ion mode. Excellent linear responses were observed with the correlation coefficients of >0.9996, and instrument detection limits (at a signal to noise of 3 : 1) were in the range of 0.30-0.33 nmol/L. The proposed method is sensitive and reproducible for the precise determination of the amino acids from wolfberry fruit and cortex lycii samples.
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; AMINO-ACIDS ; HUMAN PLASMA ; BIOGENIC-AMINES ; REAGENT ; DERIVATIZATION ; CHLOROFORMATE ; GABA ; GLUTAMATE ; SAMPLES
收录类别: SCI
语种: 英语
WOS记录号: WOS:000356593600007
Citation statistics:
内容类型: 期刊论文
URI标识: http://210.75.249.4/handle/363003/5566
Appears in Collections:中国科学院西北高原生物研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China
2.Binzhou Med Univ, Coll Pharm, Yantai, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China
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