DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT

NWIPB OpenIR

	DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT
	Sun, Zhiwei ; Xia, Lian ; Suo, Yourui ; You, Jinmao
	2010
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES ; Sun, ZW; Xia, L; Suo, YR; You, JM.DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(3):390-404
摘要	A novel fluorescent probe, 1-[1,2,5,6-dibenzocarbazol-9-yl]propan-2-yl chloroformate (DBCPC-Cl) has been designed and synthesized for amine labeling in HPLC. Using 12 aliphatic amines as the models, the derivatization conditions were optimized. In 0.2mol/L borate buffer (pH 9.0), amines reacted with DBCPC-Cl at 30 degrees C to form the derivatives in 3min, and the derivatives were stable enough to be efficiently analyzed by HPLC. The separation of these amine derivatives was achieved with a C8 column and gradient elution by using 30% acetonitrile (containing 20mmol/L formic acid) and 100% acetonitrile, and online APCI/MS identification of the derivatives was carried out in positive-ion mode. The fluorescence responses for DBCPC-derivatives were higher relative to those obtained using previously reported reagents. With fluorescence detection at an emission wavelength of 390nm and an excitation wavelength of 300nm, the detection limits of aliphatic amines were 0.3 - 5.1fmol (at a signal-to-noise ratio of 3:1). The relative standard deviations for within-day determination (n=12) were 0.051-0.079% for retention time and 0.84-1.12% for peak area for the tested aliphatic amines. The mean intra- and inter-assay precision for all amines levels were <2.52% and 3.74%, respectively. The mean recoveries ranged from 86.9 to 104.7%. Excellent linear responses were observed with coefficients >0.9991.; A novel fluorescent probe, 1-[1,2,5,6-dibenzocarbazol-9-yl]propan-2-yl chloroformate (DBCPC-Cl) has been designed and synthesized for amine labeling in HPLC. Using 12 aliphatic amines as the models, the derivatization conditions were optimized. In 0.2mol/L borate buffer (pH 9.0), amines reacted with DBCPC-Cl at 30 degrees C to form the derivatives in 3min, and the derivatives were stable enough to be efficiently analyzed by HPLC. The separation of these amine derivatives was achieved with a C8 column and gradient elution by using 30% acetonitrile (containing 20mmol/L formic acid) and 100% acetonitrile, and online APCI/MS identification of the derivatives was carried out in positive-ion mode. The fluorescence responses for DBCPC-derivatives were higher relative to those obtained using previously reported reagents. With fluorescence detection at an emission wavelength of 390nm and an excitation wavelength of 300nm, the detection limits of aliphatic amines were 0.3 - 5.1fmol (at a signal-to-noise ratio of 3:1). The relative standard deviations for within-day determination (n=12) were 0.051-0.079% for retention time and 0.84-1.12% for peak area for the tested aliphatic amines. The mean intra- and inter-assay precision for all amines levels were <2.52% and 3.74%, respectively. The mean recoveries ranged from 86.9 to 104.7%. Excellent linear responses were observed with coefficients >0.9991.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/56189
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Sun, Zhiwei,Xia, Lian,Suo, Yourui,et al. DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, Sun, ZW; Xia, L; Suo, YR; You, JM.DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(3):390-404,2010.
APA	Sun, Zhiwei,Xia, Lian,Suo, Yourui,&You, Jinmao.(2010).DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES.
MLA	Sun, Zhiwei,et al."DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2010).