Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography | |
Zou, Deng-lang1,2,3; Chen, Tao1,2; Li, Hong-mei1,2,3; Chen, Chen1,2; Zhao, Jing-yang1,2; Li, Yu-lin1,2 | |
2016-04-01 | |
发表期刊 | JOURNAL OF SEPARATION SCIENCE |
卷号 | 39期号:7页码:1278-1285 |
文章类型 | Article |
摘要 | This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography. The ultrasound-assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions. Then the crude extract was directly separated by high-speed counter-current chromatography without any pretreatment using n-hexane/ethyl acetate/methanol/water (1:7:0.5:3, v/v/v/v) as the solvent system. In 180 min, 13 mg of A, 18 mg of B, and 9 mg of C were obtained from 200 mg of crude sample. Their structures were identified as Chebulagic acid (A, 954 Da), Chebulinic acid (B, 956 Da), and Ellagic acid (C) by H-1 NMR spectroscopy. |
关键词 | Crude Extracts High-speed Counter-current Chromatography Phenolic Compounds Response Surface Methodology Terminalia Chebula Retz |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1002/jssc.201501358 |
关键词[WOS] | RESPONSE-SURFACE METHODOLOGY ; POLYPHENOLIC COMPOUNDS ; PREPARATIVE ISOLATION ; ALPHA-GLUCOSIDASE ; GALLIC ACID ; PURIFICATION ; OPTIMIZATION ; ANTIOXIDANT ; FRUITS ; ULTRAFILTRATION |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | General Program of Natural Science Foundation of Qinghai Province(2015-ZJ-908) ; Science and Technology Plan of Qinghai Province(2014-GX-C01 ; 2014-NS-515-2) |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000374029500009 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/5704 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Zou, Deng-lang,Chen, Tao,Li, Hong-mei,et al. Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(7):1278-1285. |
APA | Zou, Deng-lang,Chen, Tao,Li, Hong-mei,Chen, Chen,Zhao, Jing-yang,&Li, Yu-lin.(2016).Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,39(7),1278-1285. |
MLA | Zou, Deng-lang,et al."Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 39.7(2016):1278-1285. |
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