Fluorescent turn-off/on bioassay for hemoglobin based on dual-emission carbon nanodots-graphene oxide system with multi-detection strategies

doi:10.1016/j.aca.2016.03.048

NWIPB OpenIR

	Fluorescent turn-off/on bioassay for hemoglobin based on dual-emission carbon nanodots-graphene oxide system with multi-detection strategies
	Qu, Fei 1,2; Liu, Dongya 1,2; You, Jinmao 1,2,3
	2016-05-19
发表期刊	ANALYTICA CHIMICA ACTA
卷号	921 页码:59-66
文章类型	Article
摘要	As two members of the carbon materials family, carbon nanodots (CNDs) and graphene oxide (GO) possess many excellent optical properties resulting in a wide range of applications. In this work, the fluorescence of resultant dual-emission carbon nanodots (DECNDs) could be quenched by GO. In the presence of hemoglobin (Hb), the fluorescence would recover resulting from two interactions: one was the direct stacking effect of Hb on GO; the other one was that Hb could cover the surfaces of DECNDs; both of them would prevent the fluorescence quenching of DECNDs by GO. In the light of this mechanism, a novel fluorescent turn-off/on method has been developed for the detection of Hb based on DECNDs-GO system. By virtue of the dual emissions of these CNDs, it is noteworthy that both a single emission and ratiometric of dual emissions can be used to establish linear relationships of Hb: 0.05 -300 nM (lambda em = 386 nm), 5-500 nM (lambda em = 530 nm), and 50-500 nM (I-530/I-410), with the corresponding limit of detection (LOD) as low as 20 pM, 2 nM and 20 nM, respectively. This present system is highly selective toward Hb over other proteins and this reliable method has been successfully applied for the detection of Hb in whole blood samples. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Carbon Nanodots Dual Emissions Graphene Oxide Hemoglobin
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1016/j.aca.2016.03.048
关键词[WOS]	SENSITIVE DETECTION ; QUANTUM DOTS ; SENSOR ; NANOPARTICLES ; PROBES ; BLOOD ; CU2+ ; IONS
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China (Youth Fund Project)(21405093) ; Scientific Research Foundation of Qufu Normal University(BSQD20130117)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000374643200006
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5707
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Qu, Fei,Liu, Dongya,You, Jinmao. Fluorescent turn-off/on bioassay for hemoglobin based on dual-emission carbon nanodots-graphene oxide system with multi-detection strategies[J]. ANALYTICA CHIMICA ACTA,2016,921:59-66.
APA	Qu, Fei,Liu, Dongya,&You, Jinmao.(2016).Fluorescent turn-off/on bioassay for hemoglobin based on dual-emission carbon nanodots-graphene oxide system with multi-detection strategies.ANALYTICA CHIMICA ACTA,921,59-66.
MLA	Qu, Fei,et al."Fluorescent turn-off/on bioassay for hemoglobin based on dual-emission carbon nanodots-graphene oxide system with multi-detection strategies".ANALYTICA CHIMICA ACTA 921(2016):59-66.