Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography

NWIPB OpenIR

	Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography
	Zhao, Xiao-Hui ; Han, Fa ; Li, Yu-Lin ; Yue, Hui-Lan ; Zhao, XH (reprint author), Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810001, Peoples R China.
	2013
发表期刊	PHYTOCHEMICAL ANALYSIS ; Zhao, XH; Han, F; Li, YL; Yue, HL.Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography,PHYTOCHEMICAL ANALYSIS,2013,24(2):171-175
摘要	Introduction - Stilbene glycosides are the primary constituents of Rheum tanguticum Maxim. ex Balf., to which different bioactivities has been attributed, including: anti-HIV, anti-oxidant, anti-tumour, anti-malarial, and anti-allergy activity. However, effective methods for the isolation and purification of stilbene glycosides, such as trans-rhapontin, cis-rhapontin and trans-desoxyrhaponticin, from this herb are not currently available. Objective - To develop an efficient method for the preparative isolation and purification of three stilbene glycosides from Rheum tanguticum Maxim. ex Balf. via high-speed counter-current chromatography (HSCCC). Methods - A solvent system composed of chloroform: n-butanol: methanol: water (4:1:3:2, v/v/v/v) was developed for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. The flow rate was 1.8 mL/min. The apparatus was controlled at 800 rpm and 25 degrees C, and the effluent was monitored at 280 nm. Chemical constituents were analysed by high-performance liquid chromatography (HPLC), and their structures were identified by H-1- and C-13-NMR. Results - Under the optimised conditions, 25.5 mg trans-rhapontin, 16.0 mg cis-rhapontin and 20.5 mg trans-desoxyrhaponticin were separated from 80 mg crude sample; the isolates had purities of 99.6, 97.2 and 99.2%, respectively. Conclusion - A simple and efficient HSCCC method has been optimised for the preparative separation of stilbene glycosides from Rheum tanguticum Maxim. ex Balf. Copyright (C) 2012 John Wiley & Sons, Ltd.; Introduction - Stilbene glycosides are the primary constituents of Rheum tanguticum Maxim. ex Balf., to which different bioactivities has been attributed, including: anti-HIV, anti-oxidant, anti-tumour, anti-malarial, and anti-allergy activity. However, effective methods for the isolation and purification of stilbene glycosides, such as trans-rhapontin, cis-rhapontin and trans-desoxyrhaponticin, from this herb are not currently available.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/57468
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,et al. Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS, Zhao, XH; Han, F; Li, YL; Yue, HL.Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography,PHYTOCHEMICAL ANALYSIS,2013,24(2):171-175,2013.
APA	Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,Yue, Hui-Lan,&Zhao, XH .(2013).Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS.
MLA	Zhao, Xiao-Hui,et al."Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS (2013).