Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim | |
Han, Lijuan1,2; Suo, Yourui1,3,4; Yang, Yongjing1,2; Meng, Jing1,2; Hu, Na1,2 | |
2016-04-01 | |
发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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卷号 | 85页码:655-666 |
文章类型 | Article |
摘要 | In this study, the extraction of water-soluble polysaccharides (BDPs) from Berberis dasystachya Maxim using dynamic microwave-assisted extraction (DMAE) was discussed. A Box-Behnken design combined with response surface methodology has been employed to optimize extraction parameters of DMAE. The BDPs have been analyzed in order to identify a variety of chemical properties. Antioxidant and anti-tumor activities in vitro have been studied by DPPH, ABTS, reducing power assay, and MTT assay, respectively. The results obtained showed that the optimal extraction conditions were as follows: ratio of water to raw material (X-1) 25.84 mg/L, extraction power (X-2) 433.13 W, extraction time (X-3) 35.18 min, and the maximum yield of extraction was 6.472 +/- 0.384%, which was in good agreement with the predicted value. The physicochemical tests demonstrated that the BDPs mainly consist of rhamnose, arabinose, xylose, mannose, glucose and lactose in a molar ratio of 1:17.3:1.33:7:2.33:1.78; the average molecular weight of the BDPs was estimated to be from 2.95 x 10(5) and 1.52 x 10(3) Da, respectively. Furthermore, the BDPs exhibited effective antioxidant and anti-proliferative properties in vitro. Such pharmaceutical activities could prove useful for potential future applications involving the berries of B. dasystachya Maxim. (C) 2015 Elsevier B.V. All rights reserved. |
关键词 | B. Dasystachya Maxim Polysaccharides Response Surface Methodology Antioxidant And Anti-proliferative |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1016/j.ijbiomac.2015.10.038 |
关键词[WOS] | MICROWAVE-ASSISTED EXTRACTION ; RESPONSE-SURFACE METHODOLOGY ; WATER-SOLUBLE POLYSACCHARIDE ; ANTIOXIDANT ACTIVITIES ; STRUCTURAL-CHARACTERIZATION ; ANTICANCER ACTIVITY ; THERMAL-STABILITY ; LYCIUM-RUTHENICUM ; CHEMICAL-ANALYSIS ; IMMUNOMODULATION |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Foundation of International S&T Cooperation Program of China(S2014GR0058) |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
WOS记录号 | WOS:000370886800075 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/5785 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qinghai Univ, Xining 810001, Qinghai, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Han, Lijuan,Suo, Yourui,Yang, Yongjing,et al. Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,85:655-666. |
APA | Han, Lijuan,Suo, Yourui,Yang, Yongjing,Meng, Jing,&Hu, Na.(2016).Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,85,655-666. |
MLA | Han, Lijuan,et al."Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 85(2016):655-666. |
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