Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography | |
Yuan, Zhenzhen ; Lv, Huanhuan ; Wang, Xiaoyan ; Li, Gang ; Suo, Yourui ; Zhang, Zhang ; Wang, Honglun | |
2016 | |
发表期刊 | SEPARATION SCIENCE AND TECHNOLOGY |
摘要 | Four tannins were isolated and identified from Potentilla parvifolia using high-speed counter-current chromatography (HSCCC) in this study. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:11:1.2:11, v/v/v/v) was chosen and yielded 18.8 mg of procyanidin B3, 9.8 mg of procyanidin B6, 9.1 mg of procyanidin B7, and 6.9 mg of 2,3,4,6-tetra-O-galloyl-D-glucose, all at over 95% purity, from 120 mg of the crude sample powder. The identifications were performed with H-1 NMR and C-13 NMR. This is the first report showing the presence of procyanidin B7 and 2,3,4,6-tetra-O-galloyl-D-glucose in the genus Potentilla, and procyanidin B3 and procyanidin B6 were isolated from P. parvifolia for the first time. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/58333 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Yuan, Zhenzhen,Lv, Huanhuan,Wang, Xiaoyan,et al. Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2016. |
APA | Yuan, Zhenzhen.,Lv, Huanhuan.,Wang, Xiaoyan.,Li, Gang.,Suo, Yourui.,...&Wang, Honglun.(2016).Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography.SEPARATION SCIENCE AND TECHNOLOGY. |
MLA | Yuan, Zhenzhen,et al."Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography".SEPARATION SCIENCE AND TECHNOLOGY (2016). |
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