An advanced and universal method to high-efficiently deproteinize plant polysaccharides by dual-functional tannic acid-fe(III) complex

NWIPB OpenIR

	An advanced and universal method to high-efficiently deproteinize plant polysaccharides by dual-functional tannic acid-fe(III) complex
	Shi, Shuo; Zhang, Wentao; Ren, Xinyi; Li, Min; Sun, Jing; Li, Guoliang; Wang, Yutang; Yue, Tianli; Wang, Jianlong
	2019
发表期刊	CARBOHYDRATE POLYMERS
卷号	226
摘要	Deproteinization is a crucial step for the purification of polysaccharides from natural biomass. However, traditional deproteinization technologies often suffer from complicated operating processes and the usage of toxic chemical reagents. Herein, an advanced and universal deproteinization method based on dual-functional adsorbent consisted of preferential protein adsorption coating of tannic acid-Fe-III (TA-Fe-III) and magnetic Fe3O4 (Fe3O4@TA-Fe-III, abbreviated as FTF NPs) was developed. The proposed FTF NPs showed remarkable efficiency to remove protein from Lycium barbarum L. polysaccharides (LBPs) with deproteinization ratio of 96%, higher than the typical Sevag method (85%). Detailed adsorption kinetics studies demonstrated deproteinization process reached equilibrium after 10 min, faster than other reported deproteinization adsorbents. Furthermore, FTF NPs are structurally and functionally nondestructive as regards LBPs without using organic reagents. Also, it exhibited widespread-use deproteinization performance to several common plant species. Therefore, the proposed nano-separation based on TA-Fe-III complex is an advanced and universal tool to high-efficiently deproteinize plant polysaccharides.
关键词	Deproteinization Polysaccharides Tannic acid-Fe-III Adsorption Universal
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59672
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Shi, Shuo,Zhang, Wentao,Ren, Xinyi,et al. An advanced and universal method to high-efficiently deproteinize plant polysaccharides by dual-functional tannic acid-fe(III) complex[J]. CARBOHYDRATE POLYMERS,2019,226.
APA	Shi, Shuo.,Zhang, Wentao.,Ren, Xinyi.,Li, Min.,Sun, Jing.,...&Wang, Jianlong.(2019).An advanced and universal method to high-efficiently deproteinize plant polysaccharides by dual-functional tannic acid-fe(III) complex.CARBOHYDRATE POLYMERS,226.
MLA	Shi, Shuo,et al."An advanced and universal method to high-efficiently deproteinize plant polysaccharides by dual-functional tannic acid-fe(III) complex".CARBOHYDRATE POLYMERS 226(2019).