Determination of nitrofuran metabolites in marine products by high performance liquid chromatography-fluorescence detection with microwave-assisted derivatization

NWIPB OpenIR

	Determination of nitrofuran metabolites in marine products by high performance liquid chromatography-fluorescence detection with microwave-assisted derivatization
	Luo, Xianzhu; Sun, Zhiwei; Wang, Xu; Yu, Yanxin; Ji, Zhongyin; Zhang, Shijuan; Li, Guoliang; You, Jinmao
	2019
发表期刊	NEW JOURNAL OF CHEMISTRY
卷号	43 期号:6
摘要	A sensitive method was developed for the simultaneous detection of four nitrofuran (NF) metabolites (3-amino-2-oxazolidinone (AOZ), semicarbazide (SEM), 3-amino-morpholinomethyl-2-oxazolidinone (AMOZ) and 1-aminohydantoin (AH)) in marine products. Samples were derivatized using 7-(diethylamino)-2-oxochromene-3-carbaldehyde (DAOC) under the assistance of a microwave within 20 min, followed by analysis by high performance liquid chromatography-fluorescence detection (HPLC-FLD). These NF metabolite derivatives were completely separated on a reversed-phase Eclipse XDB-C18 column within 10 min at specific excitation and emission wavelengths (lambda(ex) = 450 and lambda(em) = 510 nm). Excellent linearity (R > 0.998) of all NF metabolite compounds was achieved with the limits of detection (LODs) and the limits of quantitation (LOQs) in the range of 0.22-0.29 mu g kg(-1) and 0.69-0.95 mu g kg(-1), respectively. Satisfactory recoveries ranging from 91.39-104.93% were obtained for all the NF metabolites. This method was successfully applied for the identification and quantitation of the NF metabolites in marine products. Furthermore, the proposed method should have powerful potential in the analysis of AOZ, AH, AMOZ and SEM in foodstuffs.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59856
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Luo, Xianzhu,Sun, Zhiwei,Wang, Xu,et al. Determination of nitrofuran metabolites in marine products by high performance liquid chromatography-fluorescence detection with microwave-assisted derivatization[J]. NEW JOURNAL OF CHEMISTRY,2019,43(6).
APA	Luo, Xianzhu.,Sun, Zhiwei.,Wang, Xu.,Yu, Yanxin.,Ji, Zhongyin.,...&You, Jinmao.(2019).Determination of nitrofuran metabolites in marine products by high performance liquid chromatography-fluorescence detection with microwave-assisted derivatization.NEW JOURNAL OF CHEMISTRY,43(6).
MLA	Luo, Xianzhu,et al."Determination of nitrofuran metabolites in marine products by high performance liquid chromatography-fluorescence detection with microwave-assisted derivatization".NEW JOURNAL OF CHEMISTRY 43.6(2019).