HPLC determination of gamma-aminobutyric acid and its analogs in human serum using precolumn fluorescence labeling with 4-(carbazole-9-yl)-benzyl chloroformate

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	HPLC determination of gamma-aminobutyric acid and its analogs in human serum using precolumn fluorescence labeling with 4-(carbazole-9-yl)-benzyl chloroformate
	Yu, Yanxin; You, Jinmao; Sun, Zhiwei; Ji, Zhongyin; Hu, Na; Zhou, Wu; Zhou, Xuxia
	2019
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	42 期号:4
摘要	In this study, a simple analytical method for the determination of gamma-aminobutyric acid, gabapentin, and baclofen by using high-performance liquid chromatography with fluorescence detection was developed. An amidogen-reactive fluorescence labeling reagent, 4-(carbazole-9-yl)-benzyl chloroformate was first used to sensitively label these analytes. The completed labeling of these analytes can be finished rapidly only within 5 min at the room temperature (25. C) to form 4-(carbazole-9-yl)-benzyl chloroformate labeled fluorescence derivatives. These labeled derivatives expressed strong fluorescence property with the maximum excitation and emission wavelengths of 280 and 380 nm, respectively. The labeled derivatives were analyzed using a reversed-phase Eclipse SB-C18 column within 10 min with satisfactory shapes. Excellent linearity (R2 > 0.995) for all analytes was achieved with the limits of detection and the limits of quantitation in the range of 0.25-0.35 and 0.70-1.10 mu g/L, respectively. The proposed method was used for the simultaneous determination of gamma-aminobutyric acid and its analogs in human serum with satisfactory recoveries in the range of 94.5-97.5%.
关键词	gamma-aminobutyric acid 4-(carbazole-9-yl)-benzyl chloroformate high-performance liquid chromatography human serum
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59863
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yu, Yanxin,You, Jinmao,Sun, Zhiwei,et al. HPLC determination of gamma-aminobutyric acid and its analogs in human serum using precolumn fluorescence labeling with 4-(carbazole-9-yl)-benzyl chloroformate[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(4).
APA	Yu, Yanxin.,You, Jinmao.,Sun, Zhiwei.,Ji, Zhongyin.,Hu, Na.,...&Zhou, Xuxia.(2019).HPLC determination of gamma-aminobutyric acid and its analogs in human serum using precolumn fluorescence labeling with 4-(carbazole-9-yl)-benzyl chloroformate.JOURNAL OF SEPARATION SCIENCE,42(4).
MLA	Yu, Yanxin,et al."HPLC determination of gamma-aminobutyric acid and its analogs in human serum using precolumn fluorescence labeling with 4-(carbazole-9-yl)-benzyl chloroformate".JOURNAL OF SEPARATION SCIENCE 42.4(2019).