On-line HPLC-DPPH bioactivity-guided assay for isolated of antioxidative phenylpropanoids from Qinghai-Tibet Plateau medicinal plant Lancea tibetica

NWIPB OpenIR

	On-line HPLC-DPPH bioactivity-guided assay for isolated of antioxidative phenylpropanoids from Qinghai-Tibet Plateau medicinal plant Lancea tibetica
	Wang, Weidong; Jiao, Lijin; Tao, Yanduo; Shao, Yun; Wang, Qilan; Yu, Ruitao; Mei, Lijuan; Dang, Jun
	2019
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷号	1106
摘要	Lancea tibetica is an important traditional Tibetan medicinal plant that grows on the Qinghai-Tibet Plateau with great development potential in pharmaceutical industry. In this study, a combinative method using HPLC-DPPH and two-dimensional liquid chromatography has been developed to identify and separate antioxidants from Lancea tibetica. Under the target-guidance of HPLC-DPPH experiment, three antioxidant fractions from Lancea tibetica were recognized. Then, separation of the three fractions using two-dimensional semi-preparation liquid chromatography led to seven phenylpropanoids: (+)-pinoresinol-beta-D-glucoside (1), isoacteoside (2), acteoside (3), tibeticoside (4),epipinoresinol (5), anthelminthicol (6) and phillygenol (7). As a result, seven major antioxidants in Lancea tibetica were isolated with more than 96% purity. Furthermore, in vitro bioassay against DPPH revealed compounds 1-7 with IC50 values ranging from 6.16 +/- 0.08 to 25.09 +/- 0.11 (mu M) and compounds 1, 2 and 3 showed activities stronger than the two reference antioxidants (vitamin C, rutin), with IC50 values of 6.16 +/- 0.08, 8.93 +/- 0.06 and 7.98 +/- 0.05 (mu M), respectively. Results of the present study indicated that the method was an efficient technique to systematically screen and isolate antioxidants from medicine crops.
关键词	Lancea tibetica HPLC-DPPH Two-dimensional Phenylpropanoids Antioxidants
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59868
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Weidong,Jiao, Lijin,Tao, Yanduo,et al. On-line HPLC-DPPH bioactivity-guided assay for isolated of antioxidative phenylpropanoids from Qinghai-Tibet Plateau medicinal plant Lancea tibetica[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1106.
APA	Wang, Weidong.,Jiao, Lijin.,Tao, Yanduo.,Shao, Yun.,Wang, Qilan.,...&Dang, Jun.(2019).On-line HPLC-DPPH bioactivity-guided assay for isolated of antioxidative phenylpropanoids from Qinghai-Tibet Plateau medicinal plant Lancea tibetica.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1106.
MLA	Wang, Weidong,et al."On-line HPLC-DPPH bioactivity-guided assay for isolated of antioxidative phenylpropanoids from Qinghai-Tibet Plateau medicinal plant Lancea tibetica".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1106(2019).