Knowledge Management System of Northwest Institute of Plateau Biology, CAS
A resonance Rayleigh scattering sensor for sensitive differentiation of telomere DNA length and monitoring special motifs (G-quadruplex and i-motif) based on the Ag nanoclusters and NAND logic gate responding to chemical input signals | |
Wang, Shuai; Qu, Fei; Han, Wenli; You, Jinmao | |
2018 | |
发表期刊 | JOURNAL OF NANOBIOTECHNOLOGY |
卷号 | 16 |
摘要 | Background: Differentiation of telomere length is of vital importance because telomere length is closely related with several deadly diseases such as cancer. Additionally, G-quadruplex and i-motif formation in telomeric DNA have been shown to act as a negative regulator of telomere elongation by telomerase in vivo and are considered as an attractive drug target for cancer chemotherapy. Results: In this assay, Ag nanoclusters templated by hyperbranched polyethyleneimine (PEI-Ag NCs) are designed as a new novel resonance Rayleigh scattering (RRS) probe for sensitive differentiation of telomere length and monitoring special motifs (G-quadruplex and i-motif). In this assay, free PEI-Ag NC probe or DNA sequence alone emits low intensities of RRS, while the formation of PEI-Ag NCs/DNA complexes yields greatly enhanced RRS signals; however, when PEI-Ag NCs react with G-quadruplex or i-motif, the intensities of RRS exhibit slight changes. At the same concentration, the enhancement of RRS signal is directly proportional to the length of telomere, and the sensitivity of 64 bases is the highest with the linear range of 0.3-50nM (limit of detection 0.12 nM). On the other hand, due to the conversion of telomere DNA molecules among multiple surrounding conditions, a DNA logic gate is developed on the basis of two chemical input signals (K+ and H+) and a change in RRS intensity as the output signal. Conclusion: Our results indicate that PEI-Ag NCs can serve as a novel RRS probe to identify DNA length and monitor G-quadruplex/i-motif through the different increasing degrees of RRS intensity. Meanwhile, the novel attributes of the nanoprobe stand superior to those involving dyes or labeled DNA because of no chemical modification, low cost, green, and high efficiency. |
关键词 | Ag nanoclusters Telomere DNA length G-quadruplex i-motif DNA logic gate |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/59953 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Wang, Shuai,Qu, Fei,Han, Wenli,et al. A resonance Rayleigh scattering sensor for sensitive differentiation of telomere DNA length and monitoring special motifs (G-quadruplex and i-motif) based on the Ag nanoclusters and NAND logic gate responding to chemical input signals[J]. JOURNAL OF NANOBIOTECHNOLOGY,2018,16. |
APA | Wang, Shuai,Qu, Fei,Han, Wenli,&You, Jinmao.(2018).A resonance Rayleigh scattering sensor for sensitive differentiation of telomere DNA length and monitoring special motifs (G-quadruplex and i-motif) based on the Ag nanoclusters and NAND logic gate responding to chemical input signals.JOURNAL OF NANOBIOTECHNOLOGY,16. |
MLA | Wang, Shuai,et al."A resonance Rayleigh scattering sensor for sensitive differentiation of telomere DNA length and monitoring special motifs (G-quadruplex and i-motif) based on the Ag nanoclusters and NAND logic gate responding to chemical input signals".JOURNAL OF NANOBIOTECHNOLOGY 16(2018). |
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