A novel high-performance liquid chromatography-fluorescence analysis coupled with in situ degradation-derivatization technique for quantitation of organophosphorus thioester pesticide residues in tea

NWIPB OpenIR

	A novel high-performance liquid chromatography-fluorescence analysis coupled with in situ degradation-derivatization technique for quantitation of organophosphorus thioester pesticide residues in tea
	Yu, Yanxin; Luo, Xianzhu; Wang, Xu; Sun, Zhiwei; Song, Cuihua; You, Jinmao
	2018
发表期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷号	410 期号:26
摘要	A novel high-performance liquid chromatography-fluorescence analysis in combination with in situ degradation-derivatization (ISD-D) technique was developed for simultaneous determination of seven organophosphorus thioester pesticides (OPTPs) in tea. The ISD-D technique was based on degradation of OPTPs by a nucleophilic substitution reaction between phenylbutane-1,2,3-trione-2-oxime and OPTPs, which can give thiol degradation products (DPs). The thiol DPs obtained were derivatized with the novel derivatization reagent N-(4-(carbazole-9-yl)-phenyl)-N-maleimide (NCPM) in a syringe. Attractively, NCPM itself did not fluoresce, whereas the derivatives of the thiol DPs fluoresced intensely, with excitation and emission maxima at 290 nm and 368 nm, respectively, which extraordinary reduced the background interference and increased the detection sensitivity for thiol DPs. Excellent linearity (R-2 > 0.995) for all OPTPs was achieved, with limits of detection and limits of quantitation ranging from 0.23 to 0.45 g/kg and from 0.75 to 1.43 g/kg, respectively. Satisfactory recoveries ranging from 90.5% to 96.0% were obtained for all OPTPs. The ISD-D technique provided a novel and sensitive strategy for quantitation of trace amounts of OPTPs in real samples.
关键词	Organophosphorus thioester pesticides Thiols High-performance liquid chromatography-fluorescence detection Tea
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59965
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yu, Yanxin,Luo, Xianzhu,Wang, Xu,et al. A novel high-performance liquid chromatography-fluorescence analysis coupled with in situ degradation-derivatization technique for quantitation of organophosphorus thioester pesticide residues in tea[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(26).
APA	Yu, Yanxin,Luo, Xianzhu,Wang, Xu,Sun, Zhiwei,Song, Cuihua,&You, Jinmao.(2018).A novel high-performance liquid chromatography-fluorescence analysis coupled with in situ degradation-derivatization technique for quantitation of organophosphorus thioester pesticide residues in tea.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(26).
MLA	Yu, Yanxin,et al."A novel high-performance liquid chromatography-fluorescence analysis coupled with in situ degradation-derivatization technique for quantitation of organophosphorus thioester pesticide residues in tea".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.26(2018).