Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a

NWIPB OpenIR

	Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a
	Li, Hui; Yang, Jiameng; Jiang, Rui; Wei, Xuefeng; Song, Chengchuang; Huang, Yongzhen; Lan, Xianyong; Lei, Chuzhao; Ma, Yun; Hu, Linyong; Chen, Hong
	2018
发表期刊	MOLECULAR THERAPY-NUCLEIC ACIDS
卷号	12
摘要	Muscle development is regulated by a series of complicate processes, and non-coding RNAs (ncRNAs) such as lncRNA have been reported to play important roles in regulating skeletal myogenesis and diseases. Here we profile the expression of lncRNA in cattle skeletal muscle tissue from fetus and adult developmental stages and detect 13,580 lncRNA candidates. Many of these lncRNAs are differentially expressed between two developmental stages. We further characterize one abundant lncRNA with the highest expression level of all downregulated lncRNAs, which we named muscle differentiation-associated lncRNA (MDNCR). Via luciferase screening, RNA binding protein immunoprecipitation (RIP), and RNA pull-down assays, MDNCR was observed to directly bind to miR-133a with 32 potential binding sites. GosB was identified as a target of miR-133a by luciferase activity, quantitative real-time qPCR, and western blotting assays. Overexpression of MDNCR increased the expression of GosB, whereas this effect was abolished by miR-133a. We found that MDNCR promotes myoblast differentiation and inhibits cell proliferation by sponging miR-133a. These results demonstrate that MDNCR binding miR-133a promotes cell differentiation by targeting GosB in cattle primary myoblasts.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59977
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Hui,Yang, Jiameng,Jiang, Rui,et al. Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a[J]. MOLECULAR THERAPY-NUCLEIC ACIDS,2018,12.
APA	Li, Hui.,Yang, Jiameng.,Jiang, Rui.,Wei, Xuefeng.,Song, Chengchuang.,...&Chen, Hong.(2018).Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a.MOLECULAR THERAPY-NUCLEIC ACIDS,12.
MLA	Li, Hui,et al."Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a".MOLECULAR THERAPY-NUCLEIC ACIDS 12(2018).