NWIPB OpenIR
Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a
Li, Hui; Yang, Jiameng; Jiang, Rui; Wei, Xuefeng; Song, Chengchuang; Huang, Yongzhen; Lan, Xianyong; Lei, Chuzhao; Ma, Yun; Hu, Linyong; Chen, Hong
2018
Source PublicationMOLECULAR THERAPY-NUCLEIC ACIDS
Volume12
AbstractMuscle development is regulated by a series of complicate processes, and non-coding RNAs (ncRNAs) such as lncRNA have been reported to play important roles in regulating skeletal myogenesis and diseases. Here we profile the expression of lncRNA in cattle skeletal muscle tissue from fetus and adult developmental stages and detect 13,580 lncRNA candidates. Many of these lncRNAs are differentially expressed between two developmental stages. We further characterize one abundant lncRNA with the highest expression level of all downregulated lncRNAs, which we named muscle differentiation-associated lncRNA (MDNCR). Via luciferase screening, RNA binding protein immunoprecipitation (RIP), and RNA pull-down assays, MDNCR was observed to directly bind to miR-133a with 32 potential binding sites. GosB was identified as a target of miR-133a by luciferase activity, quantitative real-time qPCR, and western blotting assays. Overexpression of MDNCR increased the expression of GosB, whereas this effect was abolished by miR-133a. We found that MDNCR promotes myoblast differentiation and inhibits cell proliferation by sponging miR-133a. These results demonstrate that MDNCR binding miR-133a promotes cell differentiation by targeting GosB in cattle primary myoblasts.
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/59977
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Li, Hui,Yang, Jiameng,Jiang, Rui,et al. Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a[J]. MOLECULAR THERAPY-NUCLEIC ACIDS,2018,12.
APA Li, Hui.,Yang, Jiameng.,Jiang, Rui.,Wei, Xuefeng.,Song, Chengchuang.,...&Chen, Hong.(2018).Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a.MOLECULAR THERAPY-NUCLEIC ACIDS,12.
MLA Li, Hui,et al."Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a".MOLECULAR THERAPY-NUCLEIC ACIDS 12(2018).
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