Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Anti-inflammatory Activity of Total Alkaloids from Hypecoum leptocarpum Hook. f. et Thoms | |
Wen, Huaixiu; Jiang, Lei; Zhang, Defang; Yuan, Xiang; Dang, Jun; Mei, Lijuan; Shao, Yun; Tao, Yanduo | |
2018 | |
发表期刊 | PHARMACOGNOSY MAGAZINE |
卷号 | 14期号:56 |
摘要 | Background: Hypecoum leptocarpum Hook. f. et Thoms., which is used in traditional Tibetan medicine as an antipyretic, antitussive, analgesic, and anti-inflammatory agent, contains a variety of alkaloids that could be responsible for its analgesic and anti-inflammatory properties. Objective: The present study was designed to investigate the anti-inflammatory activity of the total alkaloids from H. leptocarpum (AHL) in vitro and to elucidate the chemical structure of the anti-inflammatory components in AHL. Materials and Methods: Chemical characterization was performed using liquid chromatography/quadrupole-time-of-flight mass and diode-array detector-high performance liquid chromatography. The anti-inflammatory effects of AHL were investigated by measuring the production of inflammatory cytokines using enzyme-linked immunosorbent assay and mRNA expression by real-time polymerase chain reaction in lipopolysaccharide-induced RAW 264.7 macrophages. Results: Chemical analysis of AHL revealed the presence of seven alkaloids, protopine (13.3%), cryptopine (1.5%), leptopidinine, leptocarpine, corydamine, dihydroleptopine, and oxohydrastinine. AHL significantly suppressed the production of nitric oxide (NO), interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor-alpha (TNF-alpha) in LPS-induced RAW 264.7 cells. The maximum levels of suppression of NO, IL-1 beta, IL-6, and TNF-alpha were 86.8% +/- 2.2%, 70.1% +/- 1.5%, 100.1% +/- 2.5%, and 50.8% +/- 3.6%, respectively. IC50 values of suppression of cytokine production by AHL were 7.47 +/- 2.81 mu g/mL (NO), 0.12 +/- 0.28 mu g/mL (IL-1 beta), 0.56 +/- 0.37 mu g/mL (IL-6), and 18.95 +/- 5.23 mu g/mL (TNF-alpha). AHL was also shown to downregulate mRNA expression of inducible NO synthase, IL-1 beta, IL-6, and TNF-alpha in vitro. Conclusion: The study provides convincing evidence that AHL has strong anti-inflammatory activity. The potent activity is likely a result of synergy between the different alkaloids. |
关键词 | Analgesic anti-inflammatory Hypecoum leptocarpum Hook. f. et Thoms total alkaloids |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/60008 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Wen, Huaixiu,Jiang, Lei,Zhang, Defang,et al. Anti-inflammatory Activity of Total Alkaloids from Hypecoum leptocarpum Hook. f. et Thoms[J]. PHARMACOGNOSY MAGAZINE,2018,14(56). |
APA | Wen, Huaixiu.,Jiang, Lei.,Zhang, Defang.,Yuan, Xiang.,Dang, Jun.,...&Tao, Yanduo.(2018).Anti-inflammatory Activity of Total Alkaloids from Hypecoum leptocarpum Hook. f. et Thoms.PHARMACOGNOSY MAGAZINE,14(56). |
MLA | Wen, Huaixiu,et al."Anti-inflammatory Activity of Total Alkaloids from Hypecoum leptocarpum Hook. f. et Thoms".PHARMACOGNOSY MAGAZINE 14.56(2018). |
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