Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

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	Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers
	Zhou, Wu; Wang, Yuwei; Zhang, Gong; Luan, Guangxiang; Chen, Shasha; Meng, Jing; Wang, Honglun; Hu, Na; Suo, Yourui
	2018
发表期刊	MOLECULES
卷号	23 期号:5
摘要	The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) and a sequence characterized amplified region (SCAR) markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5-CATCCGTGCT-3) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications.
关键词	Hippophae rhamnoides L dioecious sex determination RAPD SCAR
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60017
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhou, Wu,Wang, Yuwei,Zhang, Gong,et al. Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers[J]. MOLECULES,2018,23(5).
APA	Zhou, Wu.,Wang, Yuwei.,Zhang, Gong.,Luan, Guangxiang.,Chen, Shasha.,...&Suo, Yourui.(2018).Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers.MOLECULES,23(5).
MLA	Zhou, Wu,et al."Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers".MOLECULES 23.5(2018).