NWIPB OpenIR
A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides from Sphaerophysa salsula
Wang, Weidong; Dang, Jun; Shao, Yun; Jiang, Lei; Liu, Zenggen; Mei, Lijuan; Tao, Yanduo
2020
发表期刊JOURNAL OF SEPARATION SCIENCE
卷号43期号:21
摘要Flavonoid glycosides exist widely in medicine herbs and often used as nutraceuticals because of their excellent bioactivity and low toxicity. For accurate quality control and bioactivity assessment of Sphaerophysa salsula, a rapid and productive method to isolate flavonoid glycosides is needed. Therefore, this work reports the development of a novel comprehensive strategy based on an online middle-pressure chromatography and preparative high-performance liquid chromatography for rapid enrichment and separation of flavonoid glycosides from S. salsula. First, the flavonoid glycosides were enriched using an online middle-pressure chromatographic column containing stationary middle chromatogram isolated phase. During this process, the high-volume injection of the extracting solution was realized by an empty precolumn positioned before the main chromatographic tower. Then, the compounds were separated through preparative high-performance liquid chromatography with Megress C18. As a result, one new flavonol 3-O-glycoside (2) and two known flavonol 3-O-glycosides (1, 3) were targetedly isolated from S. salsula. The content of compounds 1-3 in S. salsula was 0.09, 0.11, and 0.18 wt%, respectively. Comparing to traditional enrichment and separation methods, our technique offers significantly shorter sample pretreatment time as well as high reproducibility. We believe that our separation method has a strong potential to be used for the processing of other medicinal plants.
关键词flavonoid glycosides large volume injection online enrichment preparative chromatography traditional medicine
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/60218
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Wang, Weidong,Dang, Jun,Shao, Yun,et al. A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides from Sphaerophysa salsula[J]. JOURNAL OF SEPARATION SCIENCE,2020,43(21).
APA Wang, Weidong.,Dang, Jun.,Shao, Yun.,Jiang, Lei.,Liu, Zenggen.,...&Tao, Yanduo.(2020).A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides from Sphaerophysa salsula.JOURNAL OF SEPARATION SCIENCE,43(21).
MLA Wang, Weidong,et al."A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides from Sphaerophysa salsula".JOURNAL OF SEPARATION SCIENCE 43.21(2020).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Weidong]的文章
[Dang, Jun]的文章
[Shao, Yun]的文章
百度学术
百度学术中相似的文章
[Wang, Weidong]的文章
[Dang, Jun]的文章
[Shao, Yun]的文章
必应学术
必应学术中相似的文章
[Wang, Weidong]的文章
[Dang, Jun]的文章
[Shao, Yun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。