Tibetan Sheep Adapt to Plant Phenology in Alpine Meadows by Changing Rumen Microbial Community Structure and Function

NWIPB OpenIR

	Tibetan Sheep Adapt to Plant Phenology in Alpine Meadows by Changing Rumen Microbial Community Structure and Function
	Liu, Hongjin; Hu, Linyong; Han, Xueping; Zhao, Na; Xu, Tianwei; Ma, Li; Wang, Xungang; Zhang, Xiaoling; Kang, Shengping; Zhao, Xinquan; Xu, Shixiao
	2020
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	11
摘要	The rumen microbiota is strongly associated with host health, nutrient absorption, and adaptability. However, the composition, functioning and adaptability of rumen microbiota in Tibetan sheep (TS) across different phenological periods are unclear. In this study we used sequencing of the V4-V5 region of 16S rRNA, qPCR technology and metagenomics to investigate the adaption of rumen microbiota to forage in different stages of phenology. In a grassy period, due to the high nutritional quality of the forage, TS can produce high concentrations of NH3-N and short fatty acids by increasing the content of key bacteria in the rumen, such as Bacteroidetes, Prevotella, Succiniclasticum, Treponema, Butyrivibrio fibrisolvens, Fibrobacter succinogenes, Prevotella ruminicola, Ruminococcus albus, and Ruminococcus flavefaciens to aid in growth. In the withering period, there was a positive correlation between microorganisms which indicated the closely cooperation between microorganisms, and metagenomic analysis showed that the high genes (GHs and CBMs) and subtribe (GH8, GH12, GH45, GH6, GH9, GH5, GH10, GH3, GH52, GH11, GH57, CBM1, CBM4, CBM6, CBM16, CBM37, CBM13, CBM35, CBM42, CBM32, and CBM62) that encode cellulolytic enzymes were significantly increased when the host faced low quantity and quality of forage. Genes involved in metabolic pathways, fatty acid biosynthesis and biosynthesis of antibiotics were significantly enriched, which indicated that rumen microbiota could improve plant biomass deconstruction and energy maintenance in the face of nutritional deficiencies. In the regreen period, both the composition and function of rumen microbiota had obvious disadvantages, therefore, to improve the competitiveness of microorganisms, we suggest TS should be supplemented with high-protein feed. This study is of great significance for exploring the high altitude adaptability of TS.
关键词	Tibetan sheep rumen microbiota diversity rumen microbiota function phenological periods adaptability
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60223
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, Hongjin,Hu, Linyong,Han, Xueping,et al. Tibetan Sheep Adapt to Plant Phenology in Alpine Meadows by Changing Rumen Microbial Community Structure and Function[J]. FRONTIERS IN MICROBIOLOGY,2020,11.
APA	Liu, Hongjin.,Hu, Linyong.,Han, Xueping.,Zhao, Na.,Xu, Tianwei.,...&Xu, Shixiao.(2020).Tibetan Sheep Adapt to Plant Phenology in Alpine Meadows by Changing Rumen Microbial Community Structure and Function.FRONTIERS IN MICROBIOLOGY,11.
MLA	Liu, Hongjin,et al."Tibetan Sheep Adapt to Plant Phenology in Alpine Meadows by Changing Rumen Microbial Community Structure and Function".FRONTIERS IN MICROBIOLOGY 11(2020).