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A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides fromSphaerophysa salsula
Wang, Weidong; Dang, Jun; Shao, Yun; Jiang, Lei; Liu, Zenggen; Mei, Lijuan; Tao, Yanduo
2020
Source PublicationJOURNAL OF SEPARATION SCIENCE
AbstractFlavonoid glycosides exist widely in medicine herbs and often used as nutraceuticals because of their excellent bioactivity and low toxicity. For accurate quality control and bioactivity assessment ofSphaerophysa salsula, a rapid and productive method to isolate flavonoid glycosides is needed. Therefore, this work reports the development of a novel comprehensive strategy based on an online middle-pressure chromatography and preparative high-performance liquid chromatography for rapid enrichment and separation of flavonoid glycosides fromS. salsula. First, the flavonoid glycosides were enriched using an online middle-pressure chromatographic column containing stationary middle chromatogram isolated phase. During this process, the high-volume injection of the extracting solution was realized by an empty precolumn positioned before the main chromatographic tower. Then, the compounds were separated through preparative high-performance liquid chromatography with Megress C18. As a result, one new flavonol 3-O-glycoside (2) and two known flavonol 3-O-glycosides (1, 3) were targetedly isolated fromS. salsula. The content of compounds1-3inS. salsulawas 0.09, 0.11, and 0.18 wt%, respectively. Comparing to traditional enrichment and separation methods, our technique offers significantly shorter sample pretreatment time as well as high reproducibility. We believe that our separation method has a strong potential to be used for the processing of other medicinal plants.
Keywordflavonoid glycosides large volume injection online enrichment preparative chromatography traditional medicine
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/60229
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Wang, Weidong,Dang, Jun,Shao, Yun,et al. A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides fromSphaerophysa salsula[J]. JOURNAL OF SEPARATION SCIENCE,2020.
APA Wang, Weidong.,Dang, Jun.,Shao, Yun.,Jiang, Lei.,Liu, Zenggen.,...&Tao, Yanduo.(2020).A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides fromSphaerophysa salsula.JOURNAL OF SEPARATION SCIENCE.
MLA Wang, Weidong,et al."A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides fromSphaerophysa salsula".JOURNAL OF SEPARATION SCIENCE (2020).
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