9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine

NWIPB OpenIR

	9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine
	Sun, Luping; Zhu, Shuyun; Zheng, Zhenjia; Sun, Jing; Zhao, Xian-En; Liu, Huwei
	2020
发表期刊	JOURNAL OF CHROMATOGRAPHY A
卷号	1623
摘要	Hydroxyl-polycyclic aromatic hydrocarbons (OH-PAHs) are biomarkers for assessing the exposure levels of polycyclic aromatic hydrocarbons (PAHs). A series of stable isotope mass tags (SIMT-332/338/346/349/351/354/360/363/374/377) were firstly designed and synthesized to perform multiplexed stable isotope labeling derivatization (MSILD) of OH-PAHs in human plasma and urine. Their derivatives were enriched and purified by magnetic dispersive solid phase extraction (MDSPE) using prepared Fe3O4/GO and then determined by ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) in multiple reaction monitoring mode. 9-Plexed MSILD reagents were prepared using pipemidic acid as core structure with different isotope mass tags, in which carbonyl chloride group was used to label OH-PAHs. The SIMT-346 labeled OH-PAHs standards were used as internal standards, which can greatly increase the quantitative accuracy. 9-Plex labeled nine different real samples can be quantified by UHPLC-MS/MS in a single run. Under optimized MSILD-MDSPE conditions, good linearities of seven OH-PAHs were obtained with satisfactory coefficient of determination R-2 > 0.991. Limits of detection (LODs) of seven OH-PAHs were from 0.1 to 0.5 pg/mL, and limits of quantitation (LOQs) ranged from 0.5 to 2.0 pg/mL. The intra- and inter-day precisions ranged in 2.3-12.4% with accuracies in the range of 91.7-108.4%. Acceptable results of matrix effect (89.7-105.7%) and derivatization efficiency (> 96.4%) were obtained. In short, the developed method has been proved to be high-throughput, sensitive, accurate and easy-handling. This method was applied for the measurement of seven free OH-PAHs in human urine and plasma, and expected to provide technical support for the evaluation of PAHs exposure levels in humans. (C) 2020 Elsevier B.V. All rights reserved.
关键词	Derivatization Hydroxyl polycyclic aromatic hydrocarbons Exposome High throughput Multiplexed tags chemical isotope labeling Magnetic dispersive solid phase extraction
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60292
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Sun, Luping,Zhu, Shuyun,Zheng, Zhenjia,et al. 9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1623.
APA	Sun, Luping,Zhu, Shuyun,Zheng, Zhenjia,Sun, Jing,Zhao, Xian-En,&Liu, Huwei.(2020).9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine.JOURNAL OF CHROMATOGRAPHY A,1623.
MLA	Sun, Luping,et al."9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine".JOURNAL OF CHROMATOGRAPHY A 1623(2020).