(20S)-Protopanaxadiol Ginsenosides Induced Cytotoxicity via Blockade of Autophagic Flux in HGC-27 Cells

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	(20S)-Protopanaxadiol Ginsenosides Induced Cytotoxicity via Blockade of Autophagic Flux in HGC-27 Cells
	Han, Qingqing; Han, Lijuan; Tie, Fangfang; Wang, Zhenhua; Ma, Chengjun; Li, Ji; Wang, Honglun; Li, Gang
	2020
发表期刊	CHEMISTRY & BIODIVERSITY
卷号	17 期号:7
摘要	(20S)-Protopanaxadiol ginsenosides Rg3, Rh2 and PPD have been demonstrated for their anticancer activity. However, the underlying mechanism of their antitumor activity remains unclear. In the present study, we investigated the role of these three ginsenosides on cell proliferation and death of human gastric cancer cells (HGC-27 cells). The sulforhodamine B (SRB) assay, Western blot analysis, fluorescence microscopy, confocal microscopy, high performance liquid chromatography (HPLC) analysis, flow cytometry, and transmission electron microscopy (TEM) were used to evaluate cell proliferation, apoptosis, and autophagy. The results showed that both Rh2 and PPD were more effective than Rg3 in inhibiting HGC-27 cell proliferation and inducing cytoplasmic vacuolation, while no significant changes in apoptosis were observed. Interestingly, cytoplasmic vacuolation and blockade of autophagy flux were observed after treatment with Rh2 and PPD. Rh2 obviously up-regulated the expression of the LC3II and p62. Furthermore, the increase in lysosomal pH and membrane rupture was observed in Rh2-treated and PPD-treated cells. When HGC-27 cells were pretreated with bafilomycin A1, a specific inhibitor of endosomal acidification, cellular vacuolization was increased, and the cell viability was significantly decreased, which indicated that Rh2-induced lysosome-damage accelerated cell death. Furthermore, data derived from mitochondrial analysis showed that excessive mitochondrial reactive oxygen species (ROS) and dysregulation of mitochondrial energy metabolism were caused by Rh2 and PPD treatment in HGC-27 cells. Taken together, these phenomena indicated that Rh2 and PPD inhibited HCG-27 cells proliferation by inducing mitochondria damage, dysfunction of lysosomes, and blockade of autophagy flux. The number of glycosyl groups at C-3 position could have an important effect on the cytotoxicity of Rg3, Rh2 and PPD.
关键词	(20S)-protopanaxadiol ginsenosides autophagic flux ginsenoside Rh2 lysosome mitochondria
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60308
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Han, Qingqing,Han, Lijuan,Tie, Fangfang,et al. (20S)-Protopanaxadiol Ginsenosides Induced Cytotoxicity via Blockade of Autophagic Flux in HGC-27 Cells[J]. CHEMISTRY & BIODIVERSITY,2020,17(7).
APA	Han, Qingqing.,Han, Lijuan.,Tie, Fangfang.,Wang, Zhenhua.,Ma, Chengjun.,...&Li, Gang.(2020).(20S)-Protopanaxadiol Ginsenosides Induced Cytotoxicity via Blockade of Autophagic Flux in HGC-27 Cells.CHEMISTRY & BIODIVERSITY,17(7).
MLA	Han, Qingqing,et al."(20S)-Protopanaxadiol Ginsenosides Induced Cytotoxicity via Blockade of Autophagic Flux in HGC-27 Cells".CHEMISTRY & BIODIVERSITY 17.7(2020).