Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots

NWIPB OpenIR

	Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots
	Zhu, Shuyun; Yan, Xiaolu; Qiu, Jiayi; Sun, Jing; Zhao, Xian-En
	2020
发表期刊	SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷号	225
摘要	We describe a sensitive turn-on fluorescent assay for antioxidants by using fluorescence-tunable graphene quantum dots (GQDs). GQDs exhibited strong fluorescence without dopamine (DA). DA could self-polymerize to a thin polydopamine (PDA) film on the surface of GQDs under alkaline environment, resulting in the fluorescence quenching of GQDs via fluorescence resonance energy transfer (FRET). However, the self-polymerization of DA could be effectively inhibited in the presence of antioxidants including glutathione (GSH), ascorbic acid (AA), cysteine (Cys), and homocysteine (Hcys). Thus, the fluorescence of GQDs restored. The "turn-on" sensing of antioxidants could be achieved with high sensitivity. The detection limit for GSH, AA, Cys, and Hcys could be achieved as low as 2.4 nM, 1.5 nM, 4.2 nM, and 4.4 nM, respectively. Finally, the GQDs@PDA system was applied for monitoring cerebral antioxidants in rat brain microdialysates. This work promises new opportunities to evaluate antioxidant capacity in physiological and pathological fields. (C) 2019 Elsevier B.V. All rights reserved.
关键词	Graphene quantum dots Polydopamine Fluorescence resonance energy transfer Antioxidants Microdialysates
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60441
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhu, Shuyun,Yan, Xiaolu,Qiu, Jiayi,et al. Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2020,225.
APA	Zhu, Shuyun,Yan, Xiaolu,Qiu, Jiayi,Sun, Jing,&Zhao, Xian-En.(2020).Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,225.
MLA	Zhu, Shuyun,et al."Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 225(2020).