Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Understanding the chemical foundation and genetic mechanism of the black grain trait in quinoa by integrating metabolome and transcriptome analyses | |
Wang, Dongxia; Cao, Dong; Yao, Youhua; Wang, Jinmin; Li, Zongren; Liu, Baolong | |
2020 | |
发表期刊 | BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
![]() |
卷号 | 34期号:1 |
摘要 | Quinoa (Chenopodium quinoaWilld.) cultivars that produce black grains are thought to be more nutritious and contain powerful antioxidant properties, but little is known about the chemical foundation and the genetic mechanism behind this trait. In this research, widely-targeted metabolome and transcriptome analyses were employed to unveil the chemical and genetic differences between black and white quinoa cultivars. A total of 157 differentially expressed metabolites were found in the black and white quinoa cultivars. The tannins and flavonoids were present at high levels in the black quinoa grains. The tannins comprised 13 proanthocyanins, all of which could barely be detected in white grains. Proanthocyanidins are suggested to cause the black grain trait inArabidopsis thalianaandBrassicaspecies and are thought to produce the black grain trait, while contributing to antioxidant activity, in black grain quinoa. A total of 4620 differentially expressed unigenes were identified in the black and white quinoa grains. Structural genes associated with proanthocyanin biosynthesis, particularlyDFR,LAR,LDOXandANR, were more highly expressed in black than white grains. Genes involved in the betalain biosynthetic pathway, which competes with anthocyanin biosynthesis, showed relatively lower levels of expression in black grains. TwoMYBand oneMYCtranscription factor related to the phenylpropane metabolic pathway displayed higher expression levels in black grains, whileBvMYB1, associated with betalain biosynthesis, was not detected. This study suggested that the accumulation of proanthocyanins produces the black grain trait in quinoa, and the activation of transcription factors triggers the proanthocyanidin biosynthesis pathway. |
关键词 | Quinoa black grain metabolome transcriptome betalain flavonoid |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/60452 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Wang, Dongxia,Cao, Dong,Yao, Youhua,et al. Understanding the chemical foundation and genetic mechanism of the black grain trait in quinoa by integrating metabolome and transcriptome analyses[J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,2020,34(1). |
APA | Wang, Dongxia,Cao, Dong,Yao, Youhua,Wang, Jinmin,Li, Zongren,&Liu, Baolong.(2020).Understanding the chemical foundation and genetic mechanism of the black grain trait in quinoa by integrating metabolome and transcriptome analyses.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,34(1). |
MLA | Wang, Dongxia,et al."Understanding the chemical foundation and genetic mechanism of the black grain trait in quinoa by integrating metabolome and transcriptome analyses".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 34.1(2020). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论