Characterization of the bacterial microbiota across the different intestinal segments of the Qinghai semi-fine wool sheep on the Qinghai-Tibetan Plateau

NWIPB OpenIR

	Characterization of the bacterial microbiota across the different intestinal segments of the Qinghai semi-fine wool sheep on the Qinghai-Tibetan Plateau
	Wang, Xungang; Hu, Linyong; Liu, Hongjin; Xu, Tianwei; Zhao, Na; Zhang, Xiaoling; Geng, Yuanyue; Kang, Shengping; Xu, Shixiao
	2021
发表期刊	ANIMAL BIOSCIENCE
卷号	34 期号:12
摘要	Objective: The intestinal microbiota enhances nutrient absorption in the host and thus promotes heath. Qinghai semi-fine wool sheep is an important livestock raised in the Qinghai Tibetan Plateau; however, little is known about the bacterial microbiota of its intestinal tract. The aim of this study was to detect the microbial characterization in the intestinal tract of the Qinghai semi-fine wool sheep. Methods: The bacterial profiles of the six different intestinal segments (duodenum, jejunum, ileum, cecum, colon and rectum) of Qinghai semi-fine wool sheep were studied using 16S rRNA V3-V4 hypervariable amplicon sequencing. Results: A total of 2,623,323 effective sequences were obtained, and 441 OTUs shared all six intestinal segments. The bacterial diversity was significantly different among the different intestinal segments, and the large intestine exhibited higher bacterial diversity than the small intestine. Firmicutes, Bacteroidetes, and Patescibacteria were the dominant phyla in these bacterial communities. Additionally, at the genus level, Prevotella_1, Candidatus_ Saccharimonas, and Ruminococcaceae_UCG-005 were the most predominant genus in duodenal segment, jejunal and ileal segments, and cecal, colonic, and rectal segments, respectively. We predicted that the microbial functions and the relative abundance of the genes involved in carbohydrate metabolism were overrepresented in the intestinal segments of Qinghai semi-fine wool sheep. Conclusion: The bacterial communities and functions differed among different intestinal segments. Our study is the first to provide insights into the composition and biological functions of the intestinal microbiota of Qinghai semi-fine wool sheep. Our results also provide useful information for the nutritional regulation and production development in Qinghai semi-fine wool sheep.
关键词	High-throughput Sequencing Intestine Microbiota Qinghai Semi-fine Wool Sheep Qinghai-Tibetan Plateau
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60663
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Xungang,Hu, Linyong,Liu, Hongjin,et al. Characterization of the bacterial microbiota across the different intestinal segments of the Qinghai semi-fine wool sheep on the Qinghai-Tibetan Plateau[J]. ANIMAL BIOSCIENCE,2021,34(12).
APA	Wang, Xungang.,Hu, Linyong.,Liu, Hongjin.,Xu, Tianwei.,Zhao, Na.,...&Xu, Shixiao.(2021).Characterization of the bacterial microbiota across the different intestinal segments of the Qinghai semi-fine wool sheep on the Qinghai-Tibetan Plateau.ANIMAL BIOSCIENCE,34(12).
MLA	Wang, Xungang,et al."Characterization of the bacterial microbiota across the different intestinal segments of the Qinghai semi-fine wool sheep on the Qinghai-Tibetan Plateau".ANIMAL BIOSCIENCE 34.12(2021).